Evaluation of genotoxic effects of Apitol (cymiazole hydrochloride) in vitro by measurement of sister chromatid exchange |
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| Authors: | Zoran Stanimirovic Jevrosima Stevanovic Slobodan Jovanovic Marko Andjelkovic |
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| Institution: | aDepartment of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobodjenja 18, Belgrade 11000, Serbia and Montenegro;bDepartment of Animal Husbandry and Genetics, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobodjenja 18, Belgrade 11000, Serbia and Montenegro;cInstitute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade 11000, Serbia and Montenegro |
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| Abstract: | Apitol®, with cymiazole hydrochloride as the active ingredient, is used in bee-keeping against the ectoparasitic mite Varroa destructor. The preparation was evaluated for genotoxicity in cultured human peripheral blood lymphocytes. Sister chromatid exchange, the mitotic index and the cell proliferation index were determined for three experimental concentrations of Apitol® (0.001, 0.01 and 0.1 mg/ml). All concentrations significantly (p < 0.001) increased the mitotic index (MI = 7.35 ± 0.18%, 8.31 ± 0.20% and 12.33 ± 0.25%, respectively), the proliferative index (PI = 1.83 ± 0.01, 1.84 ± 0.01 and 1.88 ± 0.02, respectively) and the frequency of sister chromatid exchange (SCE = 8.19 ± 1.81, 8.78 ± 1.80 and 13.46 ± 1.88, respectively), suggesting that cymiazole hydrochloride has genotoxic potential. |
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| Keywords: | Apitol® (cymiazole hydrochloride) Acaricide Honey Varroa destructor Genotoxic Sister chromatid exchange |
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