Taurine chloramine modulates cytokine production by human peripheral blood mononuclear cells |
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Authors: | M Chorąży E Kontny J Marcinkiewicz W Maśliński |
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Institution: | (1) Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland, PL;(2) Department of Immunology, Jagiellonian University Medical College, Cracow, Poland, PL |
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Abstract: | Summary. The effect of taurine (Tau) and taurine chloramine (Tau-Cl) on the production of TNF-α, IL-1β, and IL-6 by peripheral blood mononuclear cells of healthy volunteers was examined. Cells were stimulated with bacterial
lipopolysaccharide (LPS) in the presence of either Tau or Tau-Cl. After 24 h culture the cytokine concentrations were measured
in both culture supernatants (secreted) and cell lysates (cell-associated) using ELISA. In LPS-stimulated cells Tau-Cl inhibited
both the secreted and cell-associated IL-1β and IL-6, while exerted dual effect on TNF-α production: raising it slightly at low and reducing at higher concentration. By contrast, Tau had no significant effect on
the cytokine production. These results indicate that Tau-Cl modulates synthesis of pro-inflammatory cytokines, and therefore
it may play a role in the initiation and propagation of immune response.
Received November 29, 2001 Accepted January 18, 2002 Published online August 30, 2002
Acknowledgments This research was supported by grants from the State Committee for Scientific Research of Poland (No 4 P05B 01018) and the
Institute of Rheumatology (No I/14). The Institute of Rheumatology is supported by a core grant from the State Committee for
Scientific Research of Poland.
Authors' address: Ewa Kontny, Ph.D., Department of Pathophysiology and Immunology, Institute of Rheumatology, Spartanska 1, 02-637 Warsaw,
Poland, E-mail: zpatiir@warman.com.pl
Abbreviations: Tau, taurine; Tau-Cl, taurine chloramine; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL-1β, interleukin 1β; IL-6, interleukin 6; PBMC, peripheral blood mononuclear cells |
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Keywords: | : Taurine chloramine TNF-α IL-1β IL-6 Peripheral blood mononuclear cells |
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