Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the <Emphasis Type="Italic">in vivo</Emphasis> metastatic potential |
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Authors: | A Lentini C Forni B Provenzano S Beninati |
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Institution: | (1) Department of Biology, University of Rome “Tor Vergata”, Rome, Italy |
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Abstract: | Summary. The in vitro and in vivo effects of two flavonons, naringenin (NG) and hesperitin (HP) on the proliferation rate of highly metastatic murine B16-F10
melanoma cell were investigated. NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation,
paralleled with both the lowering of the intracellular levels of polyamine, spermidine and spermine and the enhancement of
transglutaminase (TGase, EC 2.3.2.13) activity. Orally administered NG or HP in C57BL6/N mice inoculated with B16-F10 cells
affected the pulmonary invasion of melanoma cells in an in vivo metastatic assay. The number of lung metastases detected by a computerized image analyzer was reduced, compared to untreated
animals, by about 69% in NG-treated mice and by about 36% in HP-treated mice. Survival studies showed that 50% of the NG-treated
animals died 38 ± 3.1 days after tumor cell injection (control group: 18 ± 1.5 days) and HP-treated mice died 27 ± 2.3 days
after cell inoculation. Taken together, these findings provide further evidences for the potential anticancer properties of
dietary flavonoids as chemopreventive agents against malignant melanoma. |
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Keywords: | : Flavonoids – Melanoma – Metastasis – Transglutaminase – Polyamines |
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