Preliminary genomic characterization of microsporidian Nosema bombycis |
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Authors: | Sironmani TA |
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Institution: | (1) School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021 Tamilnadu, India |
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Abstract: | In order to characterize the genome of Nosema bombycis, the techniques of karyotyping, pulsed field gel electrophoresis, and polymerase chain reaction were applied. Nosema genomic DNA moved as 23 kb fragment on a standard agarose gel. The karyotype showed four chromosomes, the molecular karyotyping
by pulsed field gel electrophoresis also showed four chromosomes. Arbitrarily primed polymerase chain reaction (PCR) with
various primers showed amplification products of sizes ranging from 1.6 to 0.15 kb. Polymerase chain reaction with specific
primer showed an amplification product of approximately 315 nucleotides. The DNA hybridizations are discussed. This is the
first report of its kind on microsporidian Nosema bombycis. The current data can play a major role in elucidating the molecular biology of this parasite.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Bombyx mori chromosomes DNA liquid hybridization genome Microsporidia Nosema bombycis PCR amplification pulsed field gel electrophoresis silkworm |
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