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Feather degradation by Kocuria rosea in submerged culture
Authors:Vidal  L  Christen  P  Coello  MN
Institution:(1) Laboratorio de Procesos Biotecnológicos, Instituto de Biología Experimental, Universidad Central de Venezuela, AP 47279, Caracas, 1041-A, Venezuela;(2) Laboratoire de Microbiologie IRD, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France
Abstract:A strain of Kocuria rosea with keratinolytic activity was studied. In batch culture, the optimum temperature for feather degradation, bacterial growth and protease secretion was at 40 °C. A specific growth rate of 0.17 h−1 was attained in basal medium with feathers as fermentation substrate. Under these conditions, after 36 h of incubation, biomass and caseinolytic activity reached 3.2 g/l and 0.15 U/ml, respectively. Extracellular protease secretion was associated with the exponential growth phase. In batch fermentation, feather degradation up to 51% in 72 h was obtained with a conversion yield in biomass of 0.32 g/g. No organic acids were detected in the fermentation broth in significant amount. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:Biodegradation  feather            Kocuria rosea            submerged cultures
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