Aflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pests |
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Authors: | Gupta Alka Gopal Murali |
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Institution: | (1) Central Plantation Crops Research Institute, Regional Station, Kayangulam, Krishnapuram, 690533 Kerala, India |
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Abstract: | Aspergillus flavus isolated from naturally infected leaf-eating caterpillar (Opisina arenosella W.), lace bug (Stephanitis typica D.) and plant hopper (Proutista moesta Westwood), insect pests of the coconut palm, were tested for aflatoxin (AT) production by employing various media formulations.
These A. flavus isolates were earlier found to be entomopathogenic in laboratory bioassays. A laboratory contaminant and four standard aflatoxigenic
A. flavus isolates were also included in this study as reference strains. All A. flavus isolates were tested on seven AT detection media: coconut extract agar, coconut extract-sodium desoxycholate agar, coconut
extract-ascorbic acid agar, coconut extract-Czapek Dox agar, coconut extract-milk powder agar, 10% commercial coconut milk
powder agar (CCMPA) and 20% CCMPA. Only two isolates of A. flavus, originally isolated from O. arenosella and P. moesta, produced ATs. AT production was detected within 48 h of incubation and was detected continually up to 1 month. These AT-producing
A. flavus isolates also produced bright yellow pigmentation in the medium. Of all the seven media used for AT detection, CCMPA (10%)
was found to be the best one, followed by 20% CCMPA, for direct and rapid AT detection. AT production was not necessary for
pathogenicity in the insects.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Aflatoxin Aspergillus flavus coconut insect pests entomopathogenic Opisina arenosella (leaf-eating caterpillar) Proutista moesta (plant hopper) rapid detection Stephanitis typica (lace bug) |
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