Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production |
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Authors: | Zhao Jin-Fang Song Wen-Lu Cheng Jun Zhang Chuan-Xi |
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Institution: | (1) College of Agriculture and Biotechnology, Zhejiang University, 310029 Hangzhou, China;(2) State Key Lab of Clean Energy Utilization, Zhejiang University, 310027 Hangzhou, China; |
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Abstract: | The hydrogenase gene from Enterobacter cloacae (IIT-BT 08) was amplified and inserted into a prokaryotic expression vector to create a recombinant plasmid (pGEX-4T-2-Cat/hydA).
The recombinant plasmid was transformed into a hydrogen-producing strain of Enterobacter aerogenes (ATCC13408). SDS–PAGE and western blot analysis confirmed the successful expression of the GST-tagged hydA protein. Anaerobic
fermentation for the production of hydrogen from glucose was investigated using E. aerogenes ATCC13408 and the recombinant strain. The results showed that the hydrogen yield markedly increased, from 442.82 ± 22.61 ml/g
glucose in the ATCC13408 strain to 864.02 ± 36.8 ml/g glucose in the recombinant. The maximum rate of hydrogen production
was found to be 53.49 ± 3.34 ml l−1 h−1 using 1% (w/v) glucose as the substrate at pH 6.0 and a reaction temperature of 37°C. |
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