Production of copolymer poly(3-hydroxybutyrate-<Emphasis Type="Italic">co</Emphasis>-4-hydroxybutyrate) through a one-step cultivation process |
| |
Authors: | A Rahayu Z Zaleha Ahmad R M Yahya M I A Majid A A Amirul |
| |
Institution: | (1) School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;(2) Malaysian Institute of Pharmaceuticals and Nutraceuticals, MOSTI, Putrajaya, Malaysia |
| |
Abstract: | A one-step cultivation process for the production of biodegradable polymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) P(3HB-co-4HB)] by Cupriavidus sp. USMAA2-4 was carried out using various carbon sources. It was found that Cupriavidus sp. USMAA2-4 could produce approximately 44 wt.% copolymer of P(3HB-co-4HB) with 27 mol% 4HB composition when the combination of oleic acid and 1,4-butanediol are used as carbon sources in 60 h
cultivation. The manipulation of carbon-to-nitrogen ratio (C/N) resulted in the increase of dry cell weight, PHA content as
well as 4HB composition. A new strategy of introducing oleic acid and 1,4-butanediol together and separately at different
concentration demonstrated different yield in PHA content ranging from 47 to 58 wt.%. The molecular weight obtained was 234 kDa
(by adding 1,4-butanediol and oleic acid together) and 212 kDa (by adding 1,4-butanediol separately). The copolymer of P(3HB-co-4HB) produced by Cupriavidus sp. USMAA2-4 was detected statistically as a random copolymer when analysed by nuclear magnetic resonance (NMR) spectroscopy. |
| |
Keywords: | Polyhydroxyalkanoates Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) 4-Hydroxybutyrate One-step cultivation Biopolymer |
本文献已被 SpringerLink 等数据库收录! |
|