Endoglucanase Activity of Compost-Dwelling Fungus Paecilomyces inflatus is Stimulated by Humic Acids and Other Low Molecular Mass Aromatics |
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Authors: | Beata Kluczek-Turpeinen Pekka Maijala Marja Tuomela Martin Hofrichter Annele Hatakka |
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Institution: | (1) Viikki Biocenter 1, University of Helsinki, Viikinkaari 9, P.O.Box 56,00014, Finland;(2) International Graduate School Zittau, Markt 23, 02763 Zittau, Germany |
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Abstract: | Summary Paecilomyces inflatus isolated from municipal waste compost was found to have cellulolytic activity in several solid and liquid media. This study
was done to reveal the multifarious effects of municipal waste compost on endoglucanase activity of P. inflatus. The highest enzyme activities under the conditions of solid-state fermentation were measured in authentic compost samples
compared with wood, straw and bran substrates. In surface liquid cultures glucose, cellobiose, xylan, Avicel cellulose, carboxymethylcellulose
(CM-cellulose), starch and citrus pectin were used as carbon sources. All carbon sources supported the growth of P. inflatus. However, only CM-cellulose, cellobiose and pectin noticeably stimulated endoglucanase (EG) activity. Further stimulation
of EG activity was obtained in cultures containing 1% CM-cellulose as a carbon source by supplementation with low-molecular
mass aromatic compounds vanillin, veratric acid and benzoic acid, and with soil humic acid (SHA). SHA and veratric acid were
found to be the most efficient elicitors of the cellulolytic activity. P. inflatus was able to utilize nitrate and ammonium as pure nitrogen sources in media containing cellulose. |
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Keywords: | Compost endoglucanase fermentation induction Paecilomyces inflatus production |
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