Phylogenetic analysis of the bacterial community in a geothermal spring,Rupi Basin,Bulgaria |
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Authors: | Iva Tomova Margarita Stoilova-Disheva Dimitrina Lyutskanova Javier Pascual Peter Petrov Margarita Kambourova |
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Institution: | (1) Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str. 26, 1113 Sofia, Bulgaria;(2) Department of Microbiology and Ecology, University of Valencia, Av. Doctor Moliner, 50, 46100 Burjassot, Valencia, Spain;(3) Faculty of Mathematics and Informatics, Sofia University, Tsar Osvoboditel Blvd. 15, 1504 Sofia, Bulgaria; |
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Abstract: | Hot environments are between the supporting life extreme niches that appear to have maintained some degree of pristine quality
and of special biotechnological interest. Knowledge on biodiversity in terrestrial hot springs is still scanty and has not
been compared in the light of the specificity of those extreme ecological niches. Study on diversity of thermophilic bacteria
inhabiting a hot spring located in Rupi Basin (RB), South-West Bulgaria, revealed a high phylogenetic richness in it (genotypic
diversity is 0.37). A total of 120 clones were examined, and grouped in 28 phylogenetic types by their RFLP profile. 16S rRNA
gene analysis allowed the identification of nine divisions from the domain Bacteria and one Candidate division. Ten of the retrieved bacterial sequences representing one third of the sequence types showed
less than 97% similarity to the closest neighbor and were referred as new sequences. Four of them were distantly related to
validly described bacteria (showed ≤90% similarity) suggesting new taxons on at least genus level. Comparison of biodiversity
in the spring from Rupi Basin, Bulgaria with that described from other terrestrial hot springs revealed that Proteobacteria, Hydrogenobacter/Aquifex and Thermus are common bacterial groups for terrestrial hot springs. Simultaneously, specific bacterial taxons were observed in different
springs. |
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