Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain |
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Authors: | Chiu-Chung Young Ta-Chen Lin Mao-Song Yeh Fo-Ting Shen Jo-Shu Chang |
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Institution: | (1) Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung, Taiwan;(2) Department of Environmental Engineering, National Chung Hsing University, Taichung, Taiwan;(3) Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan |
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Abstract: | Summary An indigenous strain Gordonia alkanivorans CC-JG39 was isolated from oil-contaminated sludge of a local gas station located in central Taiwan. The bacterial isolate
was able to grow on diesel-containing Bushnell–Haas medium and also tolerate various chemical additives frequently used in
petroleum products (e.g. BETX, methyl-tert-butyl ether, and naphthalene). Kinetics of diesel-limited cell growth and biodegradation
of diesel followed a Monod-type model. The kinetic constants for cell growth (μmax and KS,G) were 0.158 h−1 and 3196 mg/l, respectively, while those for biodegradation of diesel (vmax, diesel and KS,D) were 3.59 mg/h/mg cell and 2874 mg/l, respectively. G. alkanivorans CC-JG39 produced extracellular surface-active material, leading to a low surface tension of nearly 33 mN/m. The CC-JG39 strain
also possessed the ability to float towards the oil/water interface. These features might play some roles in enhancing the
mass transfer efficiency between oil substrate and the bacterial cells. Therefore, G. alkanivorans CC-JG39 may have potential applications in bioremediation of oil pollution sites. |
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Keywords: | Biodegradation biosurfactant diesel oil floating activity Gordonia alkanivorans |
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