From SELDI-TOF MS to protein identification by on-chip elution |
| |
Authors: | Mæland Nilsen Mari Uleberg Kai-Erik Janssen Emiel A M Baak Jan P A Andersen Odd Ketil Hjelle Anne |
| |
Institution: | International Research Institute of Stavanger (IRIS), PB 8046, N-4068 Stavanger, Norway. mam@iris.no |
| |
Abstract: | SELDI-TOF MS has been demonstrated as a powerful tool for biomarker discovery. However, a major disadvantage of SELDI-TOF MS is the lack of direct identification of the discriminatory peaks discovered. We describe a novel experimental identification strategy where peptides/proteins captured to a weak cation exchange ProteinArray surface (CM10) are eluted, and thereafter identified by utilizing a sensitive LC-MS/MS (i.e. LTQ Orbitrap). A mixture of four known proteins was used to test the novel experimental approach described, and all four proteins were successfully identified. Additionally, a biomarker candidate previously discovered in plasma of Atlantic cod (Gadus morhua) by SELDI-TOF MS was identified. Thus, this study indicated that a combination of on-chip elution and a highly sensitive LC-MS/MS system can be an alternative approach to identify biomarker candidates discovered by use of SELDI-TOF MS. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|