Relationship between Inhibition of CO2 Fixation and Glutamine Synthetase Inactivation in Lemna gibba L. Treated with L-Methionine-D,L-Sulphoximine (MSO)

The rate of net CO₂ fixation in Lemna gibba L. was decreasedto 50% by 100–150 min incubation in the presence of 0•5mol m^–3 L-methionine-D,L-Sulphoximine (MSO), an irreversibleinhibitor of glutasnine synthetase (GS). The pattern of inhibitionwas similar in both 21% O₂ and 2% O₂. The inhibition was accompaniedby increased intracellular levels. Incubation with 10 mol m^–3 under the same conditions, but without MSO, resulted in even higher levels but the rate of CO₂ fixation was unaffected. Additions of glutamine, glutamate, glycine or serine delayedthe MSO-induced inhibition of CO₂ fixation. The same amino acidsdelayed the inactivation of GS by MSO. Thus inhibition of CO₂ fixation by MSO in Lemna is neither causedby elevated levels nor closely related to photorespiration. Possibly, MSO causes shortage of amino-N formaintenance of the functional integrity of the photosyntheticapparatus. Key words: Methionine sulphoximine, CO₂, fixation, Lemna