Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds |
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Authors: | Frias J; Bakhsh A; Jones D; Arthur A; Vidal-Valverde C; Rhodes M; Hedley C |
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Institution: | Instituto de Fermentaciones Industriales CSIC, Juan de la Cierva 3, E-28006 Madrid, Spain; Department of Applied Genetics, Department of Brassica Research, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK; Department of Food Molecular Biochemistry, Institute of Food Research Norwich Laboratory, Norwich Research Park, Norwich NR4 7UA, UK; Corresponding author; Fax: +34 1 5644853; E-mail: ifijf11@fresno.csic.es |
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Abstract: | Two lentil (Lens culinaris) cultivars, Syrian Local
Large (SLL) and PANT-L-406 (PL), have been used to study the genetics of
the raffinose family of oligosaccharides (RFO) and a related compound,
ciceritol, which is a galactosyl cyclitol. The RFO and ciceritol are the
major soluble -galactosides in lentil
seeds. Crosses were made between the two lentil lines and the patterns of
inheritance for the total -galactoside
content and for individual RFO compounds (raffinose, stachyose, verbascose)
and ciceritol were determined in the embryos and seed coats from single
seeds of the reciprocal F1s, the F2s and ten F3 families. The inheritance
patterns for each of the -galactosides were complex and much of
this complexity was attributed to an interaction between the embryo and its
surrounding testa. A clear-cut segregation pattern was observed for
verbascose. This was the result of PL embryos having very low, or no,
verbascose. The F2 embryos had a segregation ratio of 3 high to 1 low level
for this compound, suggesting that within this cross a low verbascose
content was determined by a single recessive gene. There was good evidence
from the F2 and F3 generations of a negative correlation between low levels
of verbascose and high levels of ciceritol, which suggests a metabolic link
between the RFO pathway and the pathway leading to ciceritol. The data are
discussed in terms of defining strategies for genetically manipulating
the-galactoside composition in lentil seeds, such that their
negative effects on nutrition may be overcome without reducing
significantly their positive role in abiotic stress
resistance. |
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