肠道病毒71型基因组中小干扰RNA的靶位点预测

肠道病毒71型（EV71）已经在世界范围内有过十多次大的爆发与流行，近年来EV71病毒的流行在亚洲逐渐呈上升的趋势，但是目前尚无有效的治疗措施，因此迫切需要一种治疗EV71的有效药物。本文采用生物信息学的方法，对人类EV71病毒三个不同株型（SHZH03，SHZH98和MS）RNA序列的局域二级结构进行了预测，并从这些病毒株的基因组中分别得到了长度在21～25nt的小干扰RNA靶序列碱基片段。这一结果将有助于治疗EV71药物的开发研究，对预防和控制EV71的爆发和流行也会有重要意义。

Prediction for Target Sites of Small Interfering RNA Duplexes in Enterovirus 71

With the epidemic of the human enterovirus 71 (EV71) worldwide and in the absence of any effective anti-enteroviral therapy, there is clearly a need to find a specific anti-EV71 agent. In this study, following bioinformatic method, we analyzed the local secondary structures of the RNA genome from three EV71 strains, namely, SHZH03, SHZH98 and MS. Several 21～25 base-long sequence segments in these genomes are predicted as the optimal target sites of small interfering RNA duplexes, respectively. The results will contribute to the pharmaceutical research and therapy of the epidemic intestinal virus EV71.