大黄鱼白细胞介素8基因克隆及特征分析

白细胞介素8是一种CXC型趋化性细胞因子，在免疫反应中起着非常重要的作用。本文在构建大黄鱼肌肉组织cD-NA文库的基础上，克隆了白细胞介素8基因。克隆到的白细胞介素8全长为2582bp，基因组包含106bp的5’端非编码区，52bp的外显子Ⅰ，168bp的内含子Ⅰ，133bp的外显子Ⅱ，149bp的内含子Ⅱ，87bp的外显子Ⅲ，682bp的内含子Ⅲ，13bp的外显子Ⅳ和1192bp的3’端非编码区，编码序列285bp，编码94个氨基酸。氨基酸序列具有趋化性因子CXC家族的结构特征，在进化上高度保守，与鲈鱼的同源性在90％以上。在检测的大黄鱼的10种组织中，表达量较高的为肾、肝、肠和脾，脑、心和肌肉中表达量较低。

Cloning and Characterization Analysis of Interleukin-8Gene in Larimichthys crocea

Interleukin-8 is a CXC chemokine, and plays an important role in immune reaction. On the basis of con- structing the full length eDNA library from the skeletal muscle, interleukin-8 gene was cloned and sequenced in La- rimichthys crocea. The aquired interleukin-8 gene is 2 582bp in length, including 106bp 5＇-UTR, 52bp exon Ⅰ , 168bp intron Ⅰ , 133bp exon Ⅱ , 149bp intronⅡ, 87bp exon Ⅲ, 682bp intron Ⅲ, 13bp exon Ⅳ, and 1 191bp 3＇- UTR. The coding region is 285bp which encodes 94 amino acids. The deduced amino acid sequence contains CRC domain, and is highly conserved in the evolution. The sequence similarity of Interleukin-8 between Larimichthys crocea and Lateolabrax japonicus is over 90%. In the examined ten tissues, interleukin-8 gene was expressed high- ly in kidney, liver, intestinal, and spleen, and low in heart, skeletal muscle and brain