DCLK1的空间结构和理化性质分析

DCLK1(Doublecortin-like kinase 1)是胃肠道肿瘤干细胞的分子标志物,也是一个潜在的基因治疗分子靶点,但其致癌机制和预后价值仍未被完全阐明。为了全面认识DCLK1,本文利用生物信息学手段,对DCLK1蛋白的理化性质、组织表达、亚细胞定位、信号肽、跨膜结构域、空间结构、蛋白质相互作用网络及序列同源性进行分析。结果表明人DCLK1基因编码729个氨基酸组成的蛋白,在进化过程中高度保守,属于TGS超家族和PKc_like超家族,是碱性不稳定的亲水蛋白,无信号肽和跨膜区域。该蛋白定位于细胞核的可能性最大,主要二级结构为无规卷曲。经GO分析和KEGG通路分析可知,与DCLK1相互作用的蛋白中,NOTCH蛋白、PRDM8和PRUNE2值得深入研究,预示DCLK1存在更为复杂的作用机制。本文结果为进一步研究DCLK1的功能及致癌机制提供了一定的参考。

Analysis of the spatial structure and physicochemical property of DCLK1

DCLK1(Doublecortin-like kinase 1)is a gastrointestinal cancer stem cell marker and a potential target for gene therapy, but its carcinogenic mechanism and prognostic value have not been fully elucidated. To gain insightful information of DCLK1 protein, bioinformatics methods were applied to analyze its chemical properties, tissue expression, subcellular localization, signal peptide, trans-membrane domain, space structure, protein interaction networks, and hereditary conservation.Results show that DCLK1 protein is highly conserved and comprised of 729 amino acid residues. It belongs to the TGS super family and PKc_like super family. The DCLK1 protein is alkaline, unstable, and hydrophilic without signal peptide or trans-membrane region. It is mainly located in the nucleus and the main secondary structure elements are random coil. GO analysis and KEGG pathway analysis show that among its interactive proteins, NOTCH proteins, PRDM8, and PRUNE2 deserve further study,which indicates that DCLK1 has more complex mechanism of action. The results provide some reference for further study of the function and carcinogenic mechanism of the protein.