受赤霉素调节的拟南芥F-box基因筛选分析

赤霉素(Gibberellins,GAs)作为一种植物激素,对植物的生长发育具有重要调控作用,但其作用机制有待进一步完善。F-box蛋白是SCF复合体的一个亚基,通过特异性识别底物来调控植物的生长发育。本研究采用生物信息学方法,通过分析拟南芥基因芯片数据库提供的数据筛选到38个受GA调节的候选F-box基因,并对其中6个基因进行了实时荧光定量PCR验证。Plant CARE分析显示,其中30个基因的启动子区具有GA响应元件、以及IAA、ABA、光、温度干旱胁迫、或生物钟相关的顺式作用元件。通过分析Bio Grid数据库提供的相互作用对象,发现其中18个候选F-box蛋白与GA2ox1,GA3ox1和GA3ox3具有相互作用关系。基因表达谱分析表明,这些候选F-box基因在拟南芥各个组织器官中都有不同程度的表达,对IAA、ABA、光、温度干旱等胁迫或不同光周期都具有一定的响应。为深入研究GA调控植物生长发育的分子机制提供了重要线索。

Screening and analysis of gibberellin-regulated F-boxgenes in Arabidopsis

Gibberellins (GAs) act as one plant hormone, which play an important role in the regulation of plant growth and development, but the molecular mechanism for GA-mediated plant growth regulation remains to be perfect further. F-box proteins are the subunits of SCF complex and also control the plant growth and development via the specific recognition of substrates. In this study, 38 candidate F-boxgenes regulated by GA were selected through the analysis of gene microarray data in Arabidopsisusing bioinformatics methods, and six of which were verified by real-time PCR. Furthermore, Plant CARE results show that there are thirty F-boxgenes with GA response elements and other elements involved in IAA, ABA, light, temperature and drought stresses or circadian clock in the promoter sequence. Besides, the analysis of interactions of proteins provided by BioGrid databases show that eighteen candidate F-box proteins have direct or indirect correlation with GA2ox1, GA3ox1 and GA3ox3. The gene expression profiles tell us that the candidate F-boxgenes express at all tissues and organs in Arabidopsis, and have responses to IAA, ABA, light, temperature and drought stresses, or photoperiod. Our results in this paper provide important clues for further study the molecular mechanism of plant growth and development regulated by GAs.