基于线粒体16S rRNA基因对中国狼蛛科(Lycosidae)系统发育研究

将自测的中国狼蛛科Lycosidae4亚科6属26种和从GenBank中检索到的北美2种豹蛛的mtD-NA-16S rRNA序列进行比较;以漏斗蛛科1种蜘蛛作为外群,对碱基序列的组成和遗传距离进行了分析,采用Bayesian方法和最大简约法(MP)构建分子系统树。研究结果表明:16SrRNA基因的部分序列为340bp到360bp,A T含量平均为75%,存在较强的A T含量偏向性;序列共有157个碱基存在变异,其中79个简约信息位点。狼蛛科各属间的遗传距离介于0.026￣0.200之间。2种建树方法均表明:科内的属及属内的种优先聚在一起;水狼蛛属相对马蛛属是狼蛛科中较为原始的类群,分化较早;獾蛛属作为1个单系群与熊蛛属合为1个并系,属于狼蛛亚科。狼蛛科6属间的分子系统关系为(Pirata(Hippasa(Trochsa Arctosa(Pardosa Wadicosa))))。

Phylogenetic Relationships of Chinese Wolf Spiders (Lycosidae) Inferred from 16S rRNA Sequences

The mtDNA-16S rRNA gene partial sequences(340-360 bp ) were amplified and sequenced from 26 species representing 4 subfamilies and 6 genera of Lycosidae in China. Aligning with 16S rRNA sequences of 2 species(Pardosa Lycosidae) in North America from GenBank, and selecting 1 species Agelena limbata(Agelenidae) as outgroup from China, we constructed a matrix of 29 DNA sequences. Nucleotides composition, transition and transversion, genetic distance of this segment had been analyzed. The phylogenetic trees of the Lycosidae were constructed by using the method of Bayesian and Maximum Parsimony(MP). The results indicate that average A T content of 16S rRNA gene in Lycosidae is 75 %, which shows a strong A T bias. The sequences have 157 variable sites and 79 parsimony-informative sites. Genetic distances among genera of Lycosidae are 0.026-0.200. Molecular phylogeny trees suggest that Pirata is a primordial gene of Hippasa which is differentiated earlier from other species. Trochsa is a monphyletic group, it that with Arctosa form a paraphtletic group. The molecular phylogeny of 6 genera of Lycosidae is(Pirata (Hippasa(Trochsa Arctosa(Pardosa Wadicosa)))).