探究壮方柔肝化纤颗粒对肝纤维化大鼠肝组织病理影响机制

摘要 目的：探究壮方柔肝化纤颗粒对肝纤维化大鼠肝组织病理影响机制。方法：选取80只雄性Wistar大鼠随机将其平均分成正常对照组、病理模型组、柔肝化纤颗粒低、中、高剂量组，每组各16只。四氯化碳复合因素造模，观察记录大鼠肝脏形态，采用HE染色、Masson染色观察大鼠肝组织和胶原纤维变化，检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）指标数值，分析大鼠肝组织中谷胱甘肽过氧化物酶（GSH-Px）、羟脯氨酸（HYP）、超氧化物歧化酶（SOD）与丙二醛（MDA）的含量。结果：柔肝化纤颗粒低、中、高剂量组大鼠肝细胞变性、坏死及纤维化组织增生程度均较病理模型组明显减轻。柔肝化纤颗粒低、中、高剂量组血清ALT、AST指标数值与肝组织中HYP、MDA指标水平较病理模型组呈现剂量依赖性降低（P＜0.001），而柔肝化纤颗粒高剂量组GSH-Px、SOD的指标含量显著高于病理模型组，差异均有统计学意义（P＜0.001）。结论：柔肝化纤颗粒可有效改善肝纤维化大鼠的肝细胞损害情况，减轻其肝组织炎症程度，对肝纤维化大鼠有一定的保肝护肝与抗肝纤维化作用，其机制可能与提高GSH-Px与SOD的水平、降低HYP与MDA的含量、降低氧化应激水平、调节脂质代谢、减少机体胶原蛋白合成等相关。

The Effect of Zhuangfang Rougan Huaxian Granule on Liver Histopathology in Rats with Liver Fibrosis

ABSTRACT Objective: To investigate the mechanism of Zhuangfang Rougan Huaxian Granule on liver tissue pathology in rats with liver fibrosis. Methods: Eighty male Wistar rats were randomly divided into normal control group, pathological model group, low, medium and high dose groups of Rougan Huaxian Granules, with 16 rats in each group. Carbon tetrachloride compound factors were used to make models, observe and record the morphology of rat liver, observe the changes of rat liver tissue and collagen fibers with HE staining and Masson staining, detect the values of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) indicators, and analyze the contents of glutathione peroxidase (GSH-Px), hydroxyproline (HYP), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat liver tissue. Results: The degree of liver cell degeneration, necrosis and fibrotic tissue proliferation of rats in the low, middle and high dosage groups of Rougan Huaxian granules were significantly reduced compared with those in the pathological model group. Compared with the pathological model group, the levels of serum ALT, AST, HYP and MDA in liver tissue in the low, middle and high dose groups of Rougan Huaxian Granules decreased in a dose-dependent manner (P<0.001), while the levels of GSH-Px and SOD in the high dose group of Rougan Huaxian Granules were significantly higher than those in the pathological model group(P<0.001). Conclusion: Rugan Huaxian Granule can effectively improve the damage of hepatocytes in rats with liver fibrosis, reduce the degree of inflammation of their liver tissues, and has certain liver protection and anti fibrosis effects on rats with liver fibrosis. Its mechanism may be related to increasing the level of GSH-Px and SOD, reducing the content of HYP and MDA, reducing the level of oxidative stress, regulating lipid metabolism, and reducing the synthesis of collagen in the body.