菊花总黄酮对RSV感染A549细胞趋化因子的影响

目的:探讨菊花总黄酮对小儿RSV感染A549细胞诱导RANTES及MCP-1释放作用影响。方法:实验分为细胞对照组,病毒对照组,菊花总黄酮组和病毒唑组。在Hep-2细胞和A549细胞分别加入菊花总黄酮和病毒唑的含药维持液,测定上述两种药物的最大无毒浓度;RSV病毒感染Hep-2细胞,观察药物对RSV的病毒抑制作用;RSV感染A549细胞,ELISA法测细胞趋化因子RANTES及MCP-1含量。结果:菊花总黄酮50%有效率优于病毒唑组,差异有统计学意义(P0.05);RANTES及MCP-1释放抑制作用比较中,菊花总黄酮组RANTES、MCP-1明显降低,优于病毒唑组,差异有统计学意义(P0.05)。结论:菊花总黄酮能够抑制RSV病毒活性,明显降低A549细胞释放RANTES、MCP-1,缓解患儿的呼吸道症状,对临床具有指导意义,值得临床推广。

Effects of ChrysanthemumDecoction on Chemokine of RSV Infection in A549 Cells

Objective:To investigate the effect of chrysanthemum Decoction on RANTES and MCP-1 release induced by RSV infection in A549 cells.Methods:The experiment was divided into control group, virus control group, chrysanthemum Decoction group and ribavirin group. Chrysanthemum Decoction and Ribavirin were added in Hep-2 cells and A549 cells containing maintenance medium. The maximum non-toxic concentration of the two drugs was determined; after RSV infected Hep-2 cells, chrysanthemum Decoction and virus of Polish containing maintenance solution was respectively added, and to observe the inhibitory effects of this drug for A549 cells infected with RSV. Using ELISA method, the level of cell chemokine RANTES and MCP-1 was detected.Results:Total flavonoids in chrysanthemum had an efficiency of 50 %, which was superior to ribavirin group, and the difference was statistically significant (P<0.05); RANTES and MCP-1 decreased significantly in Chrysanthemum group, and the difference was statistically significant compared with that of the ribavirin group (P<0.05).Conclusion:Chrysanthemumdecoction could inhibit RSV virus activity, reduce the release of RANTES and MCP-1 in A549 cells.