免标记荧光适体传感器的研究进展

核酸适体在治疗、诊断和生物传感等领域都引起了强烈的关注和广泛的应用。与传统的识别元素-- 抗体相比较，适体展现 出很多的优点：尺寸小，化学性质稳定，容易制备和修饰。更重要的是适体在生物传感的设计上更为灵活，因此，产生了很多高选 择性、高灵敏度的新型适体传感器。目前，很多的检测手段都被应用到适体传感器中，其中荧光的检测手段占有重要的地位。虽然 荧光适体传感器已经取得了重大的进展，但是荧光标记给传感器的设计带来很多的不便，因此，免标记的荧光适体传感器备受关 注。在本文中，我们将对免标记的荧光适体传感器的研究进展进行综述，为分析工作者发展更加灵敏、更加简单、更加应用广泛的 免标记荧光适体传感器提供依据。

Recent Advances in Label-free Fluorescence Aptasensors

Nucleic-acid aptamers have received intense attention and found wide applications in a range of areas, such as therapeutics, diagnostics and biosensors. In contrast to traditional antibodies, aptamers exhibit many advantages as molecular recognition elements in biosensors: they are small in size, chemically stable, readily available and easily modified. More importantly, aptamers offer greater flexibility in biosensor design. Therefore, many high selectivity and high sensitivity aptasensors have been reported. To date, a variety of detection techniques have been used in aptasensors, among which fluorescence detection plays an important role. Although the fluorescence aptasensors have made significant progress, the fluorescence labeling for sensor design brings a lot of inconvenience, therefore, label-free fluorescence aptasensors aroused general concern. In this paper, we summarized recent advances of the label-free fluorescence aptasensors, providing the basis for the analysis of worker to development label-free fluorescence aptasensors, which are more sensitive, more simple and more widely used.