吸入高浓度氢气对大鼠慢性阻塞性肺病的保护作用

目的:COPD是严重威胁人类健康的慢性呼吸系统疾病,而目前的治疗手段均不理想。氧化应激在COPD发病机制中发挥重要作用,而氢气具有选择性抗氧化特性。因此探讨氢气对COPD是否具有保护作用具有重要的现实意义。方法:采用被动吸烟法建立大鼠COPD模型,建模成功后对模型动物进行氢气吸入治疗。通过动物肺功能检测、肺组织形态学观察评价氢气对COPD是否具有治疗作用。并进一步通过检测血清及肺组织中MDA水平,以及肺组织中炎性因子水平初步探讨其可能的作用机制。结果:持续8周的烟熏造模,使大鼠体重Lee's指数显著降低,而氢气治疗2周后,Lee's指数较模型组有明显改善。动物肺功能检测显示,持续烟熏使大鼠肺气道阻力明显增加,潮气量和肺顺应性明显降低;氢气吸入治疗2周后,与COPD模型组相比,大鼠肺气道阻力、潮气量和肺顺应性均有明显改善。氢气可以明显改善烟熏引起的肺组织病理改变。氢气对烟熏引起血清及肺组织内MDA、TNF-α、IL-1β水平升高具有明显的抑制作用。结论:吸入氢气对烟熏引起的大鼠COPD具有明显的治疗作用。

Protective Effects of Hydrogen Inhalation on Rat Model of Chronic Obstructive Pulmonary Disease

Objective:COPD is a complex multifactorial disease, characterized by not fully reversible airflow limitation. However, until now none of the existing treatments for COPD is ideal. Oxidative stress is widely proposed as a pathogenic mechanism for COPD. Hydrogen molecules act as a new kind of selective antioxidant. So it is important to make sure if hydrogen has the protective effect on COPD.Methods:Rats were treated with hydrogen gas, after the establishment of COPD animal model by passive smoking exposure. Then pulmonary function testing and histomorphological evaluation were performed, to investigate the therapeutical effects of hydrogen on COPD. The underlying mechanisms were further studied by detecting the expression levels of MDA and proinflammatory cytokines in the serum and lung tissues.Results:The Lee''s index of rats decreased significantly after they were exposed to smoking for 8 weeks. Moreover, treatment with hydrogen for 2 weeks resulted in blocking the decrease of Lee''s index. The pulmonary function testing showed that hydrogen treatment abrogated the decrease of pulmonary function induced by smoking exposure. Moreover, hydrogen treatment improved the morphology of lung tissues damaged by smoking. Hydrogen treatment also blocked the increase of MDA, TNF-alpha and IL-1beta in the serum and lung tissues, induced by passive smoking.Conclusion:Inhalation hydrogen has protective effects on COPD induced by smoking exposure.