丙戊酸协同顺铂抑制乳腺癌和结直肠癌细胞生长

摘要 目的：探究丙戊酸（Valproic acid, VPA）协同顺铂抑制乳腺癌和结直肠癌细胞增殖。方法：首先使用Western blot 检测 VPA 对Acetyl-Histone H3蛋白水平的影响，使用Cell Counting Kit-8（CCK-8）法检测 VPA 对乳腺癌和结直肠癌细胞的细胞活力的影响。其次单药顺铂、VPA 和联合用药处理乳腺癌细胞 MDA-MB-231 和结直肠癌细胞 HCT-15，使用 IncuCyte 动态检测细胞生长过程和生长终点。结果：发现VPA 可抑制组蛋白去乙酰化酶的功能，升高Acetyl-Histone H3的蛋白水平，VPA 可抑制乳腺癌细胞和结直肠癌细胞增殖，且对 VPA 的药物敏感性相似；顺铂和 VPA 连用后可显著抑制乳腺癌和结直肠癌细胞增殖和活力。结论：本文发现 VPA 抑制组蛋白去乙酰化酶发挥抑制乳腺癌和结直肠癌细胞生长的新机制，并可以与顺铂连用提高抗肿瘤效果和药物敏感性，为同时患有癫痫和肿瘤的人群提供新的治疗思路。

Valproic Acid and Cisplatin Inhibit the Growth of Breast Cancer and Colorectal Cancer Cells

ABSTRACT Objective: To explore the inhibitory effect of Valproic acid (VPA) and cisplatin on the proliferation of breast cancer and colorectal cancer cells. Methods: Firstly, Western blot was used to detect the effects of VPA on Acetyl-Histone H3 protein levels, and Cell Counting Kit-8 (CCK-8) was used to detect the effects of VPA on the cell viability of breast cancer and colorectal cancer cells. Secondly, breast cancer cells MDA-MB-231 and colorectal cancer cells HCT-15 were treated with single drug cisplatin, VPA and a combination of drugs. IncuCyte was used to dynamically detect the cell growth process and growth endpoint. Results: It was found that VPA could inhibit the function of histone deacetylase and increase the Acetyl-Histone H3 protein level. VPA could inhibit the proliferation of breast cancer cells and colorectal cancer cells, and the drug sensitivity to VPA was similar. The combination of cisplatin and VPA can significantly inhibit the proliferation and viability of breast cancer and colorectal cancer cells. Conclusion: This study found that VPA inhibits histone deacetylase to play a new role in inhibiting the growth of breast cancer and colorectal cancer cells, and can be combined with cisplatin to improve the antitumor effect and drug sensitivity, providing a new therapeutic idea for patients with epilepsy and tumor.