担载神经生长因子(NGF)的乳液法涂层纤维体外缓释研究

目的:目前周围神经修复中,神经导管是研究热点,本文研究乳液法涂层纤维制备的神经导管在神经修复中应用的可能性。方法:本文采用乳液法制备担载NGF的丝素-聚乳酸(PLLA)涂层电纺纤维,观察纤维的形态,测定NGF的体外释放动力学参数,并考察纤维释放液对于PC12细胞增殖的影响。结果:担载NGF的涂层纤维具备类似于细胞外基质(ECM)的三维结构和多孔形态;涂层纤维中NGF体外有效缓释10天;细胞实验中,在含有释放液的培养基中生长的PC12细胞,与空白对照组相比,荧光强度平均多了2000-4000个荧光强度,所以释放液可以更好地促进PC12细胞的增殖。结论:担载NGF的乳液法涂层纺丝纤维具备促进缺损周围神经修复的条件,可以进一步研究在动物体内修复缺损周围神经中的效果,为以后的临床应用打下基础。

In Vitro Sustained Release Research of NGF Emulsion Multi-layer Electrospun Fibers

Objective:At present, nerve conduit is a hot research topic in peripheral nerve repair,We fabricated the nerve conduit made of coated fiber by emulsion method to investigate its possibility in the field of peripheral nerve repair.Methods:The NGF Silk fibroin-PLLA coated fibers was electrospun by W/O emulsion method. The morphology of the electrospun fibers was characterized and release kinetic of NGF in fibers was detected. The effect of release solution on cell activity was tested.Results:The NGF emulsion electrospun fibers had three-dimensional structure and porous shape similar to the extracellular matrix (ECM). NGF in fibers had sustained release for 10 days in vitro. The release solution could promote proliferation of PC12 cellsmore effectively compared to the control.Conclusion:NGF emulsion muli-layer electrospun fibers provide a promising approach in the application of peripheral nerve repair which make it possible in the use of clinic research.