熊果酸对酒精性骨质疏松大鼠骨形成、骨矿化的影响

目的：探讨熊果酸对酒精所致骨质疏松大鼠骨形成、骨矿化的影响。方法：雄性Wistar大鼠60只，按体重随机分为空白对照 组、熊果酸对照组、模型组、熊果酸低、中、高剂量组，同时分别给予生理盐水、150 mg/kg 熊果酸、50%酒精，50 mg/kg 熊果酸，100 mg/kg 熊果酸，150 mg/kg 熊果酸灌胃。熊果酸对照组生理盐水剂量同空白组，熊果酸低、中、高剂量组酒精剂量同模型组。灌胃共 持续8 周。磷钼酸法检测血清磷(P)含量，比色法检测血清钙(Ca)含量，酶联免疫吸附（ELISA）法检测血清骨钙素(BGP)、骨形成蛋 白-2(BMP-2)浓度；HE 染色法观察股骨结构的病理学变化。结果：与空白对照组相比较，模型组血清BGP、BMP-2 和Ca、P 均明显 降低，且有统计学差异(P < 0.05)，但熊果酸对照与空白对照组各项指标结果相近。熊果酸中、高剂量组大鼠血清BGP、Ca 和P 水 平均较模型组有显著升高，差异具有统计学意义(P < 0.05)，但仅熊果酸高剂量组血清BMP-2 显著升高(P < 0.05)。股骨组织HE 染色结果显示，空白对照组骨小梁致密、规则且较粗，粗细均匀；模型组骨小梁稀松、不规则、粗细不均匀，甚至可见骨小梁断裂； 熊果酸中、高剂量组骨小梁致密、规则、较厚、粗细均匀，未见骨小梁断裂。结论：熊果酸能够促进酒精性骨质疏松大鼠的骨形成， 抑制骨矿物质的流失，在改善酒精致骨质疏松方面有一定的保护作用。

The Influence of Ursolic Acid on Bone Formation and Bone Mineral in Alcohol-induced Osteoporosis Rats

Objective:To study influence of Ursolic Acid (UA) on bone formation and bone mineral in alcohol-induced osteoporosis in rats.Methods:Adult male wistar rats (n=10 for each group) were randomly divided into blank control group, UA control group, model group, UA low-, medium-, high group and were respectively given saline, 150 mg/kg UA, 50%alcohol, 50 mg/kg UA, 100 mg/kg UA, 150 mg/kg UA. UA control group was administered an equal volume of saline as the blank control group, and UA low-, medium-, high group were received an equal volume of alcohol as the alcohol model group. The rats were administered for 8 weeks. The content of serumphosphorus (P) was detected by phosphate method. The content of serum calcium (Ca) was detected by chromatometry method. Serumbone gla protein (BGP) and bone morphogenetic protein 2 (BMP-2) were detected by ELISA.Results:Compared with the blank control group, the contents of serumCa, P, BGP and BMP-2 were reduced in the model group (P<0.05). Compared with the model group, the contents of serum Ca, P, BGP and BMP-2 were significantly increased in the medium-, and high-dose group (P<0.05). The result of HE staining revealed that bone trabecular was rules, density and uniform thickness in blank control group, and bone trabecular was irregular, sparse, and fracture in the model group. Compared with the model group, bone trabecular was intact, arranged regularly and continuous in the medium-, and high-dose group.Conclusion:Alcohol plays its role in AOP rats by reducing bone formation and promoting bone mineral loss. UA has antiosteoporotic effects on AOP rats by promoting bone formation and reversing bone mineral loss.