| 新发甲型H1N1流感病毒HA分子的变异分析 |
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| 引用本文： | 刘琨,陈海龙,姚碧,马兴元.新发甲型H1N1流感病毒HA分子的变异分析[J].现代生物医学进展,2009,9(20). |
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| 作者姓名： | 刘琨 陈海龙 姚碧 马兴元 |
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| 作者单位： | 华东理工大学生物反应器国家重点实验室,上海,200237 |
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| 摘 要： | 目的:从分子进化水平上分析流感的起源及发展问题,研究目前爆发的H1N1病毒的HA分子的变异行为.方法:以GenBank公布的甲型流感H1N1病毒血凝素(hemagglutinin,HA)核酸序列和我国及世界范围内近几年来报告的H1N1流感病毒HA的核酸及氨基酸序列为研究对象,利用CLUSTAL 1.83和NetNGlyc 1.0等生物信息学软件对HA核酸和氨基酸序列进行了比对分析;将其糖基化位点、氨基酸序列和抗原决定簇与以往流感病毒进行了比较.同时,还将人源和猪源甲型H1N1流感病毒的HA氨基酸序列进行了序列比对和系统发育分析.结果:最新爆发的甲型H1N1流感病毒的HA除了在60,259,453,512位点高度保守区域与之前爆发的流感病毒一致外,在249位点新出现1个"-NTT-"的糖基化位点.发现所有的甲型病毒的氨基酸序列在8个氨基酸位点均发生改变,而8个氨基酸位点位于6个抗原抗原决定簇上.结论:糖基化位点的增加,氨基酸位点的改变导致抗原决定簇的改变,即抗原性漂移现象,都成为引起其传染性改变的重要原因.
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| 关 键 词： | 新发甲型流感 H1N1病毒HA分子 变异分析 |
The variation analysis of Influenza A H1N1 'HA and the initial research of pestilence |
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| LIU Kun,CHEN Hai-long,YAO Bi,MA Xing-yuan.The variation analysis of Influenza A H1N1 'HA and the initial research of pestilence[J].Progress in Modern Biomedicine,2009,9(20). |
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| Authors: | LIU Kun CHEN Hai-long YAO Bi MA Xing-yuan |
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| Institution: | LIU Kun,CHEN Hai-long,YAO Bi,MA Xing-yuan(State Key Laboratory of Bioreactor Engneering,East China University of Science and technology,shanghai 200237) |
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| Abstract: | Objective:To investigate the genesis and development of influenza in molecule level,and to investigate the variation of Influenza HA in H1N1.Method:The nucleotide sequences of the new public and recent few years' influenza A H1N1 virus' hemagglutinin(HA) from all over the world were used as analysis objects in this research.The HA's glycosylation site,antigen determinant sites,amino acid sequences were analyze by bioinformatics software-CLUSTAL 1.83 and NetNGlyc 1.0 edc.The results reveal that there is high... |
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| Keywords: | New influenza A H1N1 virus variation analysis |
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