大鼠主动脉内皮细胞的分离培养和鉴定

目的:探索大鼠主动脉原代内皮细胞体外培养方法,为体外研究提供细胞模型。方法:分离大鼠主动脉,直接贴壁于培养皿中,荧光倒置显微镜观察细胞形态,免疫组化Ⅷ因子相关抗原染色鉴定细胞。结果:约24小时组织块边缘有游离的新生细胞长出,7天即融合成片。消化传代后细胞呈短梭形或三角形,单层生长,铺路石状,Ⅷ因子表达阳性,呈指数增殖。冻存后复苏细胞活性均超过90%。结论:用贴壁法成功建立了大鼠血管内皮细胞体外培养方法,冻存细胞存活率高,为体外研究提供了稳定的模型。

Isolation Culture and Identification of Rat Aortic Endothelial Cells

Objective： To investigate the culture methods of primary rat aortic endothelial cells in vitro. Methods： Rat aorta was isolated, directly attached to the wall in a petri dish, and inverted microscope cell morphology. Immunohistochemistry factor VllI-related antigen was used to stain cells. Results： Organization block edge free new cells grow, after approximately 24, and integrated into the film 7d later. Cells were subcultured short spindle-shaped or triangular, monolayer, cobblestone, positive expression of factor VIII, the exponential proliferation. Recovery was more than 90% cell viability after cryopreservation. Conclusion： Vascular endothelial cells in vitro method was successfully established by adherent, and the survival rate of frozen cells was high, which providing a stable model for in vitro studies.