不同干预方式预防大鼠造影剂肾病的比较研究

目的:对比等渗氯化钠水化、碳酸氢钠水化、大剂量他汀、小剂量他汀、大剂量他汀加等渗氯化钠水化五种干预方式对大鼠造影剂肾病的预防效果,探讨各自的优势及不足,为临床预防造影剂肾病提供可借鉴的方法。方法:选取SD大鼠105只,随机分为7组,每组15只。A组:均为肾功能正常的健康大鼠;B组:即对照组,未给予任何干预方法;L组:采用等渗氯化钠水化干预;T组:采用碳酸氢钠水化干预;S1组:服用大剂量他汀;S2组:服用小剂量他汀;(S1+L)组:服用大剂量他汀加等渗氯化钠水化。干预48小时后测定各组大鼠的尿素氮(BUN)及肌酐(Cr)水平,并与与未给予干预组进行比较。结果:①S1、S2、(S1+L)三组的BUN和Cr水平均低于B组、L组及T组,差异具有统计学意义(P0.05);②S1组、S2组及(S1+L)组之间相互比较,BUN和Cr水平无明显差异(P0.05);③L组与T组之间比较BUN和Cr水平,差别无统计学意义(P0.05)。结论:他汀类药物预防造影剂肾病能够起到一定的作用,但其最终机制尚未明确,我们需在今后的临床实践中进一步研究证实。

Comparative Research on the Prevention of Contrast-induced Nephropathy on Rats by Different Interventions*

Objective： To discuss the advantages and disadvantages of five different interventions on the prevention of contrast-in- duced nephropathy, which including the hydration with normal saline, the hydration with sodium bicarbonate, the gavage with big dose statin, the gavage with small dose statin and the combination of the hydration with normal saline and the gavage with big dose statin, so as to provide a reference for clinical. Methods： 105 adult SD rats were selected and randomly divided into seven groups with fifteen rats in each one. Group A： Healthy rats with normal renal function; Group B： Rats without any interventions; Group L： Intervention with the isotonic sodium chloride hydration; Group T： By the hydration with sodium bicarbonate; Group Sh By the high-dosed statin; Group $2： By the low-dosed statin; Group （SI＋L）： Combination of the isotonic sodium chloride hydration and high-dosed statin. Then the levels of serum creatinine （Scr） and blood urea nitrogen （BUN） of rats in different groups were detected and compared after intervening for 48 hours. Results： （~）The levels of BUN and Scr in group S1, $2 and （SI＋L） were lower than those of the group B, group L and group T with statistically significant differences（P〈0.05）; （g）There was no significant difference about the levels of BUN and Scr in the group S1, group $2 and group （SI＋L） when comparing with each other （P〉0.05）; @There was no statistically significant difference about the levels of BUN and Scr between group L and group T （P〉0.05）. Conclusions： It is suggested that the statins could prevent the contrast-induced nephropathy. However, the ending mechanism of statins should be further studied in the clinical practices.