抗肿瘤多药耐药纳米粒的制备及其对MCF-7/ADR 细胞的体外评价

目的:肿瘤的多药耐药现象会显著降低肿瘤细胞内药物浓度,本研究通过制备抗肿瘤多药耐药的靶向给药系统来逆转肿瘤的耐药性以提升细胞对药物的敏感性,从而降低该现象对癌症治疗的阻碍。方法:本文使用乳化溶剂挥发法制备以含姜黄素两亲性嵌段共聚物载体、以紫杉醇和磁性粒为核心的抗肿瘤多药耐药纳米粒,使用透射电镜和动态粒径散射仪等对纳米粒进行表征和磁响应性测试后,使用MTT法测定纳米粒对肿瘤耐药细胞MCF-7/ADR的抑制率以探究给药系统的耐药逆转性能。结果:制备的抗肿瘤多耐药纳米粒粒径为105 nm左右,磁响应性良好。所制得载紫杉醇纳米粒包封率为74.74%,载药率为12.40%。纳米粒可以通过磁场和生物素受体介导作用促进肿瘤细胞对粒子的内化,以增加抗癌药物的蓄积。与游离紫杉醇相比,逆转细胞耐药指数达8.5。结论:纳米系统在维持自身稳定性同时,能够凭借协同作用和靶向作用较大程度提升药物对耐药肿瘤细胞的杀伤效果。

Preparation and Evaluation in Vitro of Nanoparticles Against Drug Resistant Tumor Cell MCF-7/ADR*

Objective:Tumor multi-drug resistance (MDR) can remove drugs from the interior of the tumor cells to reduce drug concentration. In our research we try to prepare targeting drug system with the property of tumor drug resistance reversal to promote the sensitivity of tumor cells to drugs which can be benefit to the cancer therapy.Methods:The research prepared nanoparticle which is consist of amphiphilic material carrier equipping curcumin, paclitaxel and magnetic particles by solvent evaporation method, then the nanoparticles were characterized by TEMand DLS, and the magnetic response property of particle was also examined. MTT method was adopted to calculate the nanoparticle cytotoxicity to multidrug resistant cancer cell MCF-7/ADR to explore the resistance reversal index of this system.Results:The size of the nanoparticle is 105 nm and it has outstanding magnetic response property. The paclitaxel-loading weight efficiency and encapsulation efficiency of the nanoparticle were determined as 12.40 % and 74.74 % respectively. Comparing with free paclitaxel, the nanoparticle can be efficiently uptaken by tumor cells from the combined effect of magnet field guided concentration and biotin receptor-mediated internalization, the resistance reversion index is 8.5.Conclusion:The stable nanosystem with the cooperative and targeting property can improve the therapeutic efficacy against drug-resistant MCF-7/ADR cells.