静电纺丝制备担载神经生长因子的聚乳酸纤维的工艺研究

目的:研究担载神经生长因子(NGF)的聚乳酸纤维乳液法静电纺丝的制备工艺,从电压、溶液浓度等工艺条件进行探索,通过扫描电镜对纤维的形态结构进行观察,旨在找到最佳纺丝制备条件,并观察该条件下纤维的体外释放行为和细胞活性。方法:将NGF水溶液分散于聚乳酸(PLLA)溶液中,通过W/O乳液法制备静电纺丝纤维。分别从电压8 k V、10 k V、12 k V,浓度梯度90mg/m L、100 mg/m L、110 mg/m L进行静电纺丝纤维的制备,对纤维的形态等进行表征。使用ELISA对NGF体外释放动力学进行检测,用Alamer Blue试剂考察纤维释放液对于PC12悬浮细胞增殖的影响。结果:浓度和电压对电纺纤维制备影响很大。当浓度过大时,易堵塞纺丝喷头且纤维弯曲,过小时纤维粗细差异较大。电压过大或过小时纤维弯曲情况严重,甚至出现缠绕现象。当浓度为100 mg/m L,电压为10 k V时制备的乳液法静电纺丝聚乳酸纤维直径粗细均匀,具有较好形态。在该条件下的制备的纤维NGF体外有效释放13天,释放液可以促进PC12细胞的增殖。结论:担载NGF的聚乳酸纤维乳液法最佳静电纺丝制备条件为:PLLA溶液浓度100 mg/m L、电压10 k V,该条件下制备的担载NGF的聚乳酸纤维体外释放可累计释放13天,其释放液可有效促进PC12细胞的增殖,为进一步研究担载NGF的聚乳酸纤维导管奠定了一定的工艺基础。

Technology Research of Electrospun PLLA Fiber Carries Nerve Growth Factor (NGF)

Objective:To explore the most suitable condition for emulsion electrospun PLLA fiber carries NGF from Voltage and concentration, and investigate its morphology and mechanical properties from release behavior in vitro and cell activity.Methods:The NGF is dispersed in a solution of polylactic acid (PLLA) solution byW/ O emulsion, voltage 8 kV, 10 kV, 12 kV and concentration 90 mg/mL, 100 mg/mL, 110 mg/mL to prepare electrospun fibers. The morphology of electrospun fibers was characterized form Electron microscopy and its kinetic release of NGF detected by ELISA kit. The effect of release solution on PC12 cells was tested by Alamer Blue.Results:Voltage and concentration have great effects on PLLA electrospun. High concentration can lead to pinhead blocking, while under low concentration the fiber curves. Voltage 10 kV, concentration 100 mg/mL, Velocity 1 mL/h is the most suitable condition for emulsion electrospun PLLA fiber carries NGF, the fiber has good morphology. NGF in fibers had sustained release for 13 days in vitro and the release solution can effectively promote the proliferation of PC12 cells.Conclusion:The most suitable condition for PLLA electrospun is voltage 10 kv, concentration 100 mg/mL, velocity 1 mL/h. Technology research of electrospun PLLA fiber carries NGF. The technology research of PLLA eletrospun carries NGF laid a technological foundation for further study about electrospun PLLA.