阿司匹林可抑制吗啡引起的HT22 细胞Wnt通路的激活

目的:研究阿司匹林对吗啡引起的Wnt通路的上调的影响。方法:在大鼠海马神经元元细胞系HT22细胞上建立了慢性吗啡成瘾模型,然后运用免疫荧光技术和Western blot技术检测wnt通路中β-catenin蛋白的表达变化变化,同时应用报告基因技术检测Wnt通路下游转录因子的活性。结果:在慢性吗啡成瘾模型组,β-catenin蛋白的表达显著增加,而在接受了6 h的阿司匹林预处理的慢性吗啡成瘾模型组,β-catenin蛋白的表达受到了显著的抑制。结论:阿司匹林可抑制吗啡引起的Wnt/β-catenin通路水平的过表达。

Morphine Induce the Activity of Wnt Signaling in HT22 Cells Which can be Inhibited by Aspirin

Objective:To analyse the role of aspirin in down-regulation the Wnt signaling which activated by morphine addiction. Many studies have revealed that Wnt signaling activated in morphine addiction rats models.Methods:Many technologies such as Immunofluorescence, western blot were carried out. We also used the Luciferase test to study the downstreamgene change in Wnt signaling after morphine addiction with or without aspirin pretreatment.Results:Many technologies such as Immunofluorescence, western blot were carried out. We also used the Luciferase test to study the downstreamgene change in Wnt signaling after morphine addiction with or without aspirin pretreatment.Conclusion:Our study identified that aspirin can inhibited the Wnt signaling which activated after chronic morphine addiction and this may be important in the cure of morphine addiction.