茶色素对人肺鳞癌细胞株SK-MES-1 细胞生长及凋亡的影响

目的:探讨茶色素对人肺鳞癌细胞株SK-MES-1细胞生长及凋亡的影响。方法:以超声细胞破碎法提取茶色素并计算得率。常规培养SK-MES-1细胞株,采用噻唑蓝溴化四唑(MTT)比色法观察不同浓度(5、2.5、1.25、0.625、0.3125 mg/m L)茶色素作用24、48 h对SK-MES-1细胞株生长的抑制作用,计算生长抑制率及IC_(50);以流式细胞术(FCM)荧光双染法(Annexin V/PI)观察茶色素对SK-MES-1细胞株凋亡的影响。结果:茶色素提取得率为7.35%。MTT实验结果显示随茶色素浓度增高和培养时间延长,其对细胞的生长抑制率也相应升高,即呈现剂量-时间依赖性,24 h和48 h处理的IC_(50)分别为2.353 mg/m L和1.494 mg/m L。流式细胞术检测作用24 h细胞凋亡,空白对照、0.625 mg/m L和1.25 mg/m L剂量组的SK-MES-1细胞凋亡率分别为7.7%、20.37%和25.25%。结论:茶色素可促进SK-MES-1细胞凋亡并抑制其增殖。

Effects of Tea Pigments on the Growth and Apoptosis in Lung Squamous Carcinoma Cells SK-MES-1

Objective:To study the effect of tea pigment on the growth and apoptosis of human lung squamous cell carcinoma cell line SK-MES-1.Methods:Tea pigments were extracted by ultrasonic cell disintegration, and the yield was calculated. SK-MES-1 cells were conventionally cultured. MTT assay was used to detect the inhibition ratio of different concentrations of tea pigments (5, 2.5, 1.25, 0.625, 0.3125 mg/mL) for different times (24 h and 48 h) in SK-MES-1 cells, the growth inhibition ratio (IR) and IC50 were calculated. Flow cytometry (FCM) stained with by Annexin V and IP was applied to detect the effects of tea pigments on the apoptosis of SK-MES-1 cells.Results:The extraction rate of tea pigments was 7.35 %. The results of MTT assay demonstrated a dose-time dependent manner. That is, with the increase of concentration and extension of action time, the inhibition rate of SK-MES-1 was gradually increased. IC50 of cells treated for 24 h and 48 h were 2.353 mg/mL and 1.494 mg/mL respectively. FCM technique detected the apoptosis of cells when they were treated for 24 h. The cell apoptosis ratio of control group, low and high concentration of tea pigment were 7.7 %, 20.37 %and 25.25%respectively.Conclusion:Tea pigments could promote the apoptosis of SK-MES-1 cells and inhibit its proliferation.