| MMP1 在炎症大鼠牙髓组织中表达 |
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| 引用本文： | 刘妍,伊丹,张娇,仲晓飞,王薇.MMP1 在炎症大鼠牙髓组织中表达[J].现代生物医学进展,2016,16(3):438-441. |
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| 作者姓名： | 刘妍 伊丹 张娇 仲晓飞 王薇 |
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| 作者单位： | 沈阳军区总医院口腔科；沈阳和平美晶口腔门诊部；沈阳沈河松露口腔门诊部；解放军463 医院口腔科 |
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| 基金项目： | 国家自然科学基金项目（31400820） |
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| 摘 要： | 目的:探讨基质蛋白酶MMP-1在炎症性牙髓中的表达。方法:内毒素制备大鼠牙髓炎模型,取牙髓炎大鼠及健康大鼠牙髓组织行HE染色检测牙髓组织的组织形态学变化,MMP-1特异性免疫组织化学染色和Western blot等检测MMP-1在牙髓组织的表达,以明确MMP-1在大鼠急性牙髓炎期的空间分布改变及蛋白表达量变化。结果:大鼠牙髓组织急性炎症期牙髓炎模型中可见大量炎性细胞浸润,结缔组织有断裂破坏。免疫组织化学分析及Western Blot分析均显示牙髓炎组织MMP-1蛋白显著增高(P0.05)。结论:MMP-1在牙髓炎组织中高表达,可能参与调节牙髓炎症反应的疼痛机制调节。
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| 关 键 词： | 牙髓 基质金属蛋白酶 炎症 牙痛 |
Expression of MMP1 in Rat Pulpitis |
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| Abstract: | Objective:To investigate the expression of matrix metalloproteinases MMP-1 in rat pulp tissues and its role in inflammatory
pain of pulpitis.Methods:LPS was used to construct rat pulpitis model. Hematoxylin-eosin staining was carried out to observe the
morphological changes in pulp tissues. Then the expression of MMP-1 protein in dental pulp of both pulpitis group and normal control
group were tested by immunohistochemistry and Western blot analysis.Results:Inflammatory cell infiltration was found in rat model of
pulpitis, and the connective tissues had disruption. Immunohistochemistry and Western blot analysis showed that MMP-1 protein level
was significantly higher in pulpitis group than that of control group (P<0.05).Conclusion:MMP-1 overexpression in pulpitis may have
an important role in the pain mechanismof inflammatory response to pulpitis. |
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| Keywords: | Dental pulp Matrix metalloproteinase Inflammation Toothache |
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