白藜芦醇通过Pi3K/AKT 通路抑制胃癌SGC-7901 细胞增殖和迁移

目的:验证白藜芦醇是否可以抑制胃癌SGC-7901细胞增殖和迁移及其信号通路。方法:用不同浓度白藜芦醇干预SGC-7901细胞,再用LY-294002和IGF-1分别用来抑制和激活Pi3K/AKT通路。MTT法测细胞增殖,划痕试验和Transwell试验测细胞迁移,Western blot检测细胞迁移相关蛋白(MMP-2、MMP-9)、细胞迁移相关蛋白(P21、P27)、以及AKT、p-AKT的表达情况;结果:相比于对照组,白藜芦醇组胃癌细胞增殖和迁移减弱(P=0.001),p-AKT表达减少(P0.001);LY-294002可以抑制p-AKT的表达(P=0.004),和白藜芦醇一样可以抑制胃癌细胞的增殖和迁移;IGF-1可以显著增加p-AKT的表达(P0.001),可以逆转白藜芦醇对胃癌细胞增殖和迁移的抑制作用。结论:白藜芦醇通过抑制Pi3K/AKT信号通路抑制胃癌细胞增殖和迁移。

Resveratrol Inhibit Gastric Carcinoma Cell SGC-7901 Proliferation and Migration by Inhibiting Pi3K/AKT Pathway

Objective:To verify whether resveratrol could inhibit SGC-7901 cells proliferation and migration through Pi3K/AKT pathway.Methods:Different concentrations of resveratrol were used to incubate with SGC-79011 cells. LY-294002 and IGF-1 were used to inhibit and activate Pi3K/AKT pathway respectively. MTT were used to investigate the proliferation of SGC-7901 cells. Transwell chambers and wound healing were employed to test the migratory ability of SGC-79011 cells. Western blotting were used to investigate the expressions of P21, P27, MMP-2, MMP-9, AKT and p-AKT inSGC-7901 cells.Results:Compared with the control group, the proliferation and migration of SGC-7901 cells and the expression of p-AKT were decreased in the resveratrol group (P=0.001); the inhibitory effect of the downregulation of p-AKT by LY-294002on the induction of SGC-7901 cells proliferation and migration was identical to that of resveratro (P<0.001); upregulation of p-AKT by IGF-1reversed the resveratrol-induced inhibition of SGC-7901 cells dedifferentiation (P<0.001).Conclusion:Resveratrol inhibits SGC-7901 cells proliferation and migration by suppressing Pi3K/AKT signaling pathway.