| 磷脂酰肌醇3激酶抑制剂LY294002对缺血缺氧心肌细胞的作用研究 |
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| 引用本文： | 宋华培,颜洪,党永明,褚志刚,张琼,黄跃生.磷脂酰肌醇3激酶抑制剂LY294002对缺血缺氧心肌细胞的作用研究[J].现代生物医学进展,2007,7(11):1659-1661. |
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| 作者姓名： | 宋华培 颜洪 党永明 褚志刚 张琼 黄跃生 |
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| 作者单位： | 第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆,400038 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;军队专项课题 |
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| 摘 要： | 目的:明确P13K/Akt信号通路在缺血缺氧心肌细胞损害中的作用。方法:建立心肌细胞缺血缺氧模型,施加磷脂酰肌醇3激酶抑制剂LY294002干预,观察心肌细胞活力、培养液中乳酸脱氢酶(LDH)含量及碘化丙啶(PI)染色阳性细胞比例的变化。结果:模拟缺血缺氧后细胞活力下降,LDH及PI染色阳性细胞比例显著增加。LY294002干预复合缺血缺氧后,细胞活力急剧下降,LDH含量及PI染色阳性细胞比例进一步显著增加(P<0.01)。结论:应用LY294002加重了缺血缺氧对心肌细胞的损伤效应,提示PI3K/Akt通路参与了缺血缺氧心肌细胞的内源性保护反应,减轻了缺血缺氧损害。
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| 关 键 词： | 心肌细胞 磷脂酰肌醇3激酶 缺血缺氧 |
| 文章编号： | 1673-6273（2007）11-1659-03 |
| 修稿时间： | 2007-08-02 |
Effects of LY294002, a Specific Inhibitor of Phosphatidylinositol 3 Kinase,on Cardiomyocytes Subject to Ischemia and Hypoxia |
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| SONG Hua-pei,YAN Hong,DANG Yong-ming,CHU Zhi-gang,ZHANG Qiong,HUANG Yue-sheng.Effects of LY294002, a Specific Inhibitor of Phosphatidylinositol 3 Kinase,on Cardiomyocytes Subject to Ischemia and Hypoxia[J].Progress in Modern Biomedicine,2007,7(11):1659-1661. |
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| Authors: | SONG Hua-pei YAN Hong DANG Yong-ming CHU Zhi-gang ZHANG Qiong HUANG Yue-sheng |
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| Institution: | Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combinedlujury,bird Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective:To clarify the effects of PI3K/Akt pathway on cardiomyocytes subjected to ischemia and hypoxia.Methods: Cardiomyocytes were cultured in vitro.Viabilities of cardiomyocytes,lactate dehydrogenase(LDH)leaking and ratio of propidium io- dide(PI)staining positive cells were observed to test the effects of LY294002,a specific inhibitor of phosphatidylinositol 3-kinase,when exposed to stimulation of isehemia and hypoxia.Results:Viabilities of cardiomyocytes subjected to ischemia and hypoxia by the inhibi- tion of PI3K/Akt pathway were significantly lower and LDH leak-age and ratio of PI staining positive cells were significantly higher than those without PI3K/Akt pathway inhibition(P<0.01 ).Conclusion:LY294002 can aggravate the injury of cardiomyocytes subjected to is- ehemia and hypoxia,which suggests that the PI3K/Akt pathway can protect cardiomyocytes from injury of ischemia and hypoxia. |
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| Keywords: | LY294002 |
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