非小细胞肺癌组织中VEGF与P53蛋白表达及意义

目的:探讨VEGF和P53在非小细胞肺癌(NSCLC)中的表达及其生物学行为的关系。方法:采用免疫组织化学方法,对病理确诊的62例非小细胞肺癌组织进行VEGF和P53表达的检测。结果:62例非小细胞肺癌VEGF阳性表达率58.1%(36/62),其表达与NSCLC的组织分化程度、生存期有相关性(P<0.05),与组织学类型、有无淋巴结转移、临床病理分期无关。P53阳性表达率46.8%(29,62),表达与肿瘤组织类型、TNM分期、有无淋巴结转移无关(P<0.05)。结论:VEGF和P53可作为评价非小细胞肺癌预后的重要指标。用免疫组化检测出P53蛋白可间接反映P53基因的突变。本组资料检测的结果显示,P53基因阳性表达率46.8% (29/62),表明近一半肺癌病例已失去P53蛋白的抑癌功能,且P53基因的突变可能与非小细胞肺癌的发生有关。P53和VEGF都是潜在的新型肿瘤表型标志物,它们的检测可作为肿瘤病理分级诊断的主要参考依据。同时检测P53和VEGF的表达状态,可通过肿瘤血管形成的生物学行为信息,进一步研究肿瘤血管形成的分子水平调控机制,这有助于抗肿瘤药物的开发研究。

Expression and Significance of VEGF and P53 in Non Small Cell Lung Cancer

Objective:To study the expression of VEGF and p53 proteins in non small cell lung cancer(NSCLC)and the relation- ship with biological behavior of NSCLC.Methods:Expression of the VEGF and p53 proteins was evaluated by immunohistochemical polymer detection systemmethod in 62 cases of non small cell lung cancer.Results:The positive expression rate of VEGF was 58.1% (36/62)in 62 cases of non small cell lung cancer,which is related to histological differentiation and surviving time (P<0.05).There was no significant correlation between VEGF and pathological type,lymph node metastases status and clinical stage(P>0.05).The positive expression rate of p53 was 46.8%(29/62) in this study,which has no correlation between p53 and pathological type clinical stage and lymph node metastases status(P>0.05).Conclusions:Determining VEGF and p53 protein expressions simultaneously may play an im- portant role in judgment of prognosis of non small cell lung cancer,p53 mutation can be reflected indirectly by immunohistochemical polymer detection system method.Our study shows that the positive expression rate of p53 is 46.8%.That is to say,1/2 cases of lung can- cer have lost the function of restraining cancer,which shows that the mutation of p53 may be related to the development of NSCLC.Both p53 and VEGF new markers of tumor phenotype.Simultaneous detecting p53 and VEGF become the main reference of pathological di- agnosis.Further study will likely clarify the molecular mechanism of tumor angiogenesis.It is also helpful to the development of anti-tu- mor medicines.