| 双脱甲氧基姜黄素对SKOV3卵巢癌细胞抑制作用的体外实验研究 |
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| 引用本文： | 段海霞,马向东,王胜春,胡咏武,赵辉平.双脱甲氧基姜黄素对SKOV3卵巢癌细胞抑制作用的体外实验研究[J].现代生物医学进展,2010,10(6). |
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| 作者姓名： | 段海霞 马向东 王胜春 胡咏武 赵辉平 |
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| 作者单位： | 1. 第四军医大学西京医院妇产科,陕西,西安,710032
2. 西京医院药剂科,陕西,西安,710032 |
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| 摘 要： | 目的:研究双脱甲氧基姜黄素体外抑制SKOV3卵巢癌细胞抑制作用的体外实验研究。方法:(1)将不同浓度双脱甲氧基姜黄素(0、10ug/ml、20ug/ml、40ug/ml)作用于人卵巢癌SKOV3细胞6、12、24h,采用四甲基偶氮唑盐(MTT)比色法检测SKOV3细胞的增殖活性;(2)流式细胞仪观察双脱甲氧基对SKOV3卵巢癌细胞周期的作用,免疫组化检测增殖细胞核抗原(PCNA)的表达。结果:(1)双脱甲氧基姜黄素对SKOV3细胞具有增殖抑制作用,且具有浓度和时间依赖效应;(2)双脱甲氧基姜黄素能诱导SKOV3细胞G1期阻滞,通过抑制细胞生长周期,降低细胞增生速率抑制肿瘤细胞增殖;(3)双脱甲氧基姜黄素能下调SKOV3细胞PCNA蛋白表达,并呈一定的浓度依赖性。结论:双脱甲氧基姜黄素能明显抑制人卵巢癌SKOV3细胞的体外增殖,可能为临床治疗卵巢癌提供了一种新的方法。
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| 关 键 词： | 双脱甲氧基姜黄素(BDC) 卵巢癌细胞SKOV3 细胞增殖 |
Inhibition Effect of Bisdemethoxycurcumin on SKOV3 Ovarian Cancer Cells in vitro |
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| DUAN Hai-xia,MA Xiang-dong,WANG Sheng-chun,HU Yong-wu,ZHAO Hui-ping.Inhibition Effect of Bisdemethoxycurcumin on SKOV3 Ovarian Cancer Cells in vitro[J].Progress in Modern Biomedicine,2010,10(6). |
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| Authors: | DUAN Hai-xia MA Xiang-dong WANG Sheng-chun HU Yong-wu ZHAO Hui-ping |
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| Institution: | DUAN Hai-xia1,MA Xiang-dong1,WANG Sheng-chun2,HU Yong-wu2,ZHAO Hui-ping3 (1 Department of Gynecology , Obstetrics,Xijing Hospital,Fourth Military Medicine University,Xi\'an 710032,China,2 Department of Pharmacy X ijing Hospital,The Fourth Military Medical University,Xi\'an 710033,China) |
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| Abstract: | Objective: To investigate the effect and mechanism (bisdemethoxycurcumin,BDC inhibiting SKOV3 cells in vitro.Methods: 1(.bisdemethoxycurcumin,BDC)of different concent rations (0 ,10 ,20 and 40ug/ml ) was administered to SKOV3 cells of ovarian cancer f or 6h ,12h and 24 h ,respectively.The proliferation of SKOV3 cells was measured by MTT assay.2.Induction of apoptosis was detected with FCM.3.Protein expressions of PCNA were detected with immunohistochemistry;Results: (1) (bisdemethoxycurcumin,BDC) inhibited ... |
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| Keywords: | (bisdemethoxycurcumin BDC) SKOV3 proliferation |
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