乌头须根总生物碱提取工艺的考察

目的:考察乌头须根中总生物碱的最佳提取工艺条件。方法:酸碱滴定法测定乌头须根中总生物碱含量,以总生物碱提取率为指标,采用L9(3)~4正交实验法筛选乌头须根总生物碱的最佳提取工艺。结果:乌头须根总生物碱含量为1.094%,影响提取的主次因素为:乙醇浓度>提取次数>提取时间>乙醇用量;优选得到的最佳提取工艺为A_3B_1C_3D_3,即以8倍量80%的乙醇提取3次,每次1.5小时。结论:乌头须根总生物碱含量较高,提取工艺条件稳定、经济、可行。

Study on the Extract Technology of Total Alkaloids from Fibrous Root of Aconitum carmichaeli

Objective:To determine the extract technology of total alkaloids from the fibrous root of Aconitum carmichaeli Debx. Methods:The method of acid-alkali titrations was applied to determine the contents of total alkaloids,the optimum conditions were investigated by orthogonal design and the extraction ratio of total alkaloids was used as the index.Results:The contents of total alkaloids from the fibrous root of Aconitum was 1.094%,factors influencing the extracting efficiency were as follow:the concentration of alcohol>the frequency of extract>the time of extracting>the quantity of Alcohol;the optimum extracting condition was A_3B_1C_3D_3,that was adding eight times amount of 80% alcohol and refluxing and extracting for 3 h (totally extracting for 3 times).Conclusions:The contents of total alkaloids from the fibrous root of Aconitum carmichaeli is high,and the extracting condition has good reproducibility, and it is workable,economic and convenient.