Obestatin对血浆ghrelin和nesfatin-1水平及胃排空的影响

目的:探讨侧脑室注射obestatin对大鼠血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平的影响以及对胃排空的调控。方法:侧脑室注射obestatin,采用酶免疫测定(EIA)法检测血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平以及胃排空率的变化。结果:侧脑室分别注射0.1、0.3或1.0 nmol obestatin,大鼠血浆酰基化ghrelin、去酰基化ghrelin以及nesfatin-1水平无显著改变(P0.05),且酰基化ghrelin与去酰基化ghrelin比率无显著改变(P0.05);侧脑室注射obestatin,大鼠摄食量无显著改变,但胃排空率明显增加(P0.05);胃排空率明显延迟(P0.05)。与侧脑室注射1.0 nmol Obestatin组相比,注射1.0 nmol Obestatin+CRF,大鼠摄食量无显著改变,胃排空率明显延迟(P0.05)。各组摄食量及进入十二指肠内食物量无明显差异(P0.05)。结论:中枢obestatin促进大鼠的胃排空,可能与h/r CRF通路有关。

The Effects of Obestatin on Plasmaacyl Gheelin and Nesfatin-1 Levels, and on Gastric Emptying in Rats

Objective:To discuss the impact of intracerebroventricular obestatin on plasma acyl ghrelin, des-acyl ghrelin and nesfatin-1 levels, and the regulation of gastric emptying in rats.Methods:Rats were administrated obestatin through intracerebroventricular (ICV) injection; Enzyme immunoassay (EIA) method was used to detect the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 and changes in the rate of gastric emptying.Results:After lateral ventricle injection respectively 0.1, 0.3 or 1.0 nmol obestatin, the plasma levels of acyl ghrelin, des-acyl ghrelin, nesfatin-1 had no significant change (P>0.05), and the ratio of acyl ghrelin and des-acyl ghrelin had no significant difference (P>0.05) too. Food intake of rats had no obvious difference after lateral ventricle injection of obestatin, but the gastric emptying rate increased significantl (P<0.05). Gastric emptying rate delayed significantly (P<0.05). Compared with lateral ventricle injection 1.0 nmol Obestatin group, injection 1.0 nmol Obestatin + CRF, no significant changes in food intake in rats significantly delayed gastric emptying rate (P<0.05). While the amount of food intake and the food into the duodenum had no obvious difference (P>0.05).Conclusion:Central obestatin promotes gastric emptying of rats, which may be associated with h/rCRF pathways.