肺结核患者血清IFN-γ、Il-1β和TNF-α水平的临床检测价值分析

目的:研究肺结核患者血清γ干扰素(IFN-γ)、白介素-1β(Il-1β)以及肿瘤坏死因子-α(TNF-α)水平的临床检测价值。方法:选择2015年1月～2018年12月在北京市顺义区医院治疗的25例肺结核患者作为肺结核组,并且选择同期在该院进行体检的25例健康人作为对照组。采用酶联免疫吸附法(ELISA)检测并且比较肺结核组以及对照组研究对象的血清IFN-γ、Il-1β和TNF-α水平,比较痰菌阴性组(n=14例)以及痰菌阳性组(n=11例)、无空洞组(n=15例)以及有空洞组(n=10例)的血清IFN-γ、Il-1β、TNF-α水平。结果:肺结核组患者的血清IFN-γ、Il-1β、TNF-α水平均明显高于对照组(P0.05);痰菌阳性组肺结核患者的血清IFN-γ、Il-1β、TNF-α水平均明显高于痰菌阴性组患者(P0.05);有空洞组肺结核患者的血清IFN-γ、Il-1β、TNF-α水平均明显高于无空洞组患者(P0.05)。结论:肺结核患者的血清IFN-γ、Il-1β和TNF-α水平明显高于健康者,有助于判断疾病进程,这些细胞因子可能在结核病的发病中发挥着重要的作用。

Clinical Value of Serum IFN-gamma, Il-1beta and TNF-alpha Levels for the Patients with Pulmonary Tuberculosis

ABSTRACT Objective: To study the clinical value of serum interferon-gamma (IFN-gamma), interleukin-1beta (Il-1beta) and tumor necrosis factor-alpha (TNF-alpha) levels in patients with pulmonary tuberculosis. Methods: 25 tuberculosis patients treated in Shunyi District Hospital of Beijing from January 2015 to December 2018 were selected as the tuberculosis group, and 25 healthy people who underwent physical examination in the hospital during the same period were selected as the control group. The serum levels of IFN-gamma, Il-1beta and TNF-alpha in tuberculosis group and control group were detected and compared by ELISA. The serum levels of IFN-gamma, Il-1beta and TNF-alpha in sputum negative group (n=14 cases) and sputum positive group (n=11 cases), non-cavity group (n=15 cases) and cavity group (n=10 cases) were compared. Results: The serum levels of IFN-gamma, Il-1beta and TNF-alpha in tuberculosis group were significantly higher than those in control group (P < 0.05). The serum levels of IFN-gamma, Il-1beta and TNF-alpha in sputum positive group were significantly higher than those in sputum negative group (P < 0.05). The serum levels of IFN-gamma, Il-1beta and TNF-alpha in patients with pulmonary tuberculosis in the cavity group were significantly higher than those in the non-cavity group (P < 0.05). Conclusion: The serum levels of IFN-gamma, Il-1beta and TNF-alpha in patients with pulmonary tuberculosis were significantly higher than those in healthy people. These cytokines may help to judge the course of disease and play an important role in the pathogenesis of tuberculosis.