血卟啉单甲醚介导的声动力疗法对牙龈卟啉单胞菌生物膜中丙二醛含量的影响

目的:探讨血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)介导的声动力疗法(sonodynamic therapy,SDT)对牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)生物膜中脂质过氧化物丙二醛(malondialdehyde,MDA)含量的影响。方法:羟基磷灰石片培养Pg生物膜厌氧培养3天,将生物膜随机分为4组(对照组、HMME组、超声组、SDT组),分别与无菌生理盐水或HMME进行避光孵育,然后进行声动力处理。采用平板计数法计算细菌存活率,MDA含量使用MDA检测试剂盒在可见光分光光度计下进行检测。结果:当超声强度为3 W/cm~2,超声时间为5 min时,SDT组的生物膜细菌存活率仅为40%,与对照组相比显著降低(P0.05),超声组细菌存活率为62%,与对照组相比亦显著降低(P0.05)。相同超声参数下,SDT组Pg生物膜中MDA含量最高,达17.3±1.2 nmol/mL(P0.05),超声组生物膜中MDA含量为7±0.8 nmol/mL,与对照组相比差异并无统计学意义(P0.05)。结论:HMME介导的SDT对Pg生物膜有一定杀伤效果,并且在杀伤过程中,可引发脂质过氧化反应,导致MDA释放。

Effect of Sonodynamic Therapy with Hematoporphyrin Monomethyl Ether on the MDA content in the Porphyromonas Gingivalis Biofilm

ABSTRACT Objective: To investigate the effects of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on the content of lipid peroxides malondialdehyde (MDA) in Porphyromonas gingivalis (Pg) biofilm. Methods: Hydroxyapatite tablets were used to culture Pg biofilm anaerobic for 3 days, then biofilm were randomly divided into 4 groups (control group, HMME group, ultrasound group and SDT group), incubated with sterile saline or HMME respectively, and then treated with ultrasound. The bacteria survival rate was calculated by plate counting method. MDA content was detected by MDA detection kit using visible light spectrophotometer after ultrasound treatment. Results: When the ultrasound intensity was 3 W/cm2 and the ultrasound duration was 5 min, the survival rate of bacteria in the SDT group was only 40% (P<0.05), compared to the control group. The survival rate of bacteria in the ultrasonic group decreased to 62% (P<0.05) after the ultrasound treatment, compared to the control group. In the same ultrasound parameters, MDA content was the highest in SDT group, up to 17.3±1.2 nmol/mL (P<0.05), while the content of MDA ultrasound group was 7±0.8 nmol/mL, which was not significant statistically compared to the control group (P>0.05). Conclusion: HMME mediated SDT has a certain killing effect to Pg biofilm, and during the killing process, lipid peroxidation was induced, leading to the release of MDA.