白藜芦醇对急性痛风性关节炎大鼠的影响(英文)

目的:观察白藜芦醇对急性痛风性关节炎大鼠的影响。方法:选取Wista大鼠36只,随机分为正常对照组、模型组、秋水仙碱组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组,各组相应采用生理盐水、秋水仙碱、白藜芦醇低、中、高剂量灌胃7天(1次/日),模型组及各实验组于灌胃第四天把25g/mL(0.05mL)浓度的尿酸盐溶液注射到大鼠踝关节腔内,制备急性痛风性关节炎模型,正常对照组大鼠关节腔内注射生理盐水0.05 ml,72 h后留取踝关节关节液及关节滑膜,应用ELISA法观察关节液中IL-1β、CXCL10的变化。关节滑膜用10%福尔马林固定待做病理。结果:与模型组比较,白藜芦醇能显著降低关节液中IL-1β、CXCL10水平(P0.05),病理结果显示,白藜芦醇可减轻急性痛风性关节炎大鼠踝关节组织的水肿和炎性细胞浸润。结论:急性痛风性关节炎发病过程中IL-1β,CXCL10明显增高,白藜芦醇可有效抑制急性痛风性关节炎发作,且该作用呈一定的剂量依赖性。关键词:痛风性关节炎;白藜芦醇;白介素1-β

The Effect of Resveratrol on Rats Acute Gouty Arthritis*

Objective： To investigate the effects ofresveratrol on rats acute gouty arthritis. Methods： According to random number table, 36 wista rats were divided into six groups. Control group, model group, Colchicine group, high dose Resveratrol group（HR）, medium dose Resveratrol group （MR）, low dose Resveratrol group （LR）, were administrated of normal saline, Colchicine suspension, high dose Resveratrol, medium dose Resveratrol, low dose Resveratrol, respectively, once a day for 7days. Rats acute gouty arthritis modles were estalished on the fourth day by monosodium urate solution （concentration of 25 mg/mL, 0.05 mL） injected into the ankle cavity, while 0.05 mL normal saline was injected into joint cavity of rats in control group. 72 hours later, model establishment synovial fluid was col- lected from ankel to detect level ofIL-1β, CXCL10. Synovium were fixed to do Histopathologic with 10% formalin. Results： The level ofIL-1 β, CXCL10 in resveratrol group were lower than that in model group （P〈0.05）. The Histopathologic results showed that resvera- trol might reduce acute gouty arthritis joint edema, improve inflammatory cells infiltrating. Conclusion： The levels of IL- 1β and CX- CL10 were raised in acute gouty arthritis. The resveratrol can effectively control acute gouty arthritis attack ,and the curative effect is dose dependent.