磷脂分析方法的研究进展

磷脂是细胞膜的重要结构组成成分,是许多生理活性物质的前体物质,还发挥着细胞信号传导,细胞增殖,细胞凋亡等作用。研究表明许多疾病与磷脂的代谢异常有关,对磷脂的分析有助于疾病的诊断和治疗,对磷脂的研究已经成为医学,生物和药学等众多学科的研究热点,因为磷脂结构复杂,种类繁多,对磷脂的分析一直比较困难,随着分析技术的不断进步,特别是质谱技术,文献报道应用HPLC-ESI-MS进行磷脂分析的报道也越来越多,为了更好地认识磷脂的结构,功能及其代谢过程等,本文从磷脂的提取方法,色谱分析,质谱检测方法和代谢组学研究等四个方面对对近年来针对磷脂分析的研究进展加以综述。

The Recent Advances in Analysis of Phospholipids*

Phospholipids is major constituents of cell membrane, and also plays an important role in cellular signal transduction, cell proliferation and apoptosis, and as a precursor substance of many physiologically relevant molecules. Recent researches have shown that phospholipids abnormal metabolism are closely related to the aggravation of many diseases. The analysis of phospholipids is very important for the diagnosis and the treatment of disease. More and more attention have been paid to the annalysis ofphospholipids. It is diffcult to analyse the phospholipids due to the complex structure and species of Phospholipids. With the progress of analysis technology, especially, the mass spectrometry, more and more attenntion have been paid on the analysis of phospholipid by using HPLC-ESI-MS. In order to understand the function and metabolism of phospholipids better, the research of the extraction method, the chromatographic analysis, mass spectrometry and metabolomics of phospholipids were stmunarized. In this article, the recent advances of analysis of phospholipids are reviewed.