碳/碳材料含硅羟基磷灰石涂层体外成骨细胞相容性研究

目的:成骨细胞在碳/碳复合材料表面羟基磷灰石涂层上有良好的长入,该实验在羟基磷灰石(hydroxyapatite HA)涂层中掺入硅后,研究成骨细胞对该涂层的生物活性,为临床骨科应用提供实验基础。方法:本研究采用化学液相气化沉积/水热法在碳/碳材料表面制备了含硅羟基磷灰石(silicon-hydroxyapatite Si-HA)涂层。在体外成骨细胞相容性的研究中,以HA涂层为对照,通过甲基噻唑基四唑(methylthiazolyl tetrazolium MTT)法测定细胞增殖反应和对细胞毒性反应,碱性磷酸酶(alkaline phosphatase ALP)测定细胞的分化,扫描电镜观察细胞生长形态,免疫荧光显影技术测定细胞的长入。结果:在HA涂层中引入硅后,Si-HA涂层和HA涂层在第2天、第4天的成骨细胞增殖实验表明前者的细胞长入数较后者多,两者的差异有统计学意义,而Si-HA涂层浸泡液中的ALP活性下降较HA涂层而言更明显,两者的差异有统计学意义,电镜扫描及荧光染色均提示在Si-HA涂层中成骨细胞的增值数更多。结论:在HA涂层中引入硅后,改变了涂层自身的晶体结构及表面电荷,同时诱导成骨细胞分泌胶原,使得成骨细胞更好的贴壁生长和增殖,成骨能力增加。在临床骨科植入物表面涂层改性上有很好的应用前景。

In Vitro Study of the Osteoblast Compatibility of Silicon Hydroxyapatite Coatings on Carbon/carbon Material*

Objective： Osteoblasts have good growth in hydroxyapatite coating on carbon/carbon composites. To evaluate the biological activity of osteoblasts to the hydroxyapatite coating includes silicon in this study, it may provide an experimental basis for clinical orthopedic applications. Methods： In this study, silicon hydroxyapatite （Si-HA） coating on C/C material was made by the way of chemical liquid-vaporized deposition / hydrothermal. In vitro cytocompatibility study, as the control of HA coating by MTT method for the determination of cell proliferation and cytotoxicity, Alkaline phosphatase for the cell differentiation, scanning electron microscopy for cell morphology, immunofluorescence imaging techniques for cell ingrowth. Remits： After the introduction of silicon in the HA coating, osteoblast proliferation experiment showed that the number of cells growed into Si-HA coating more than HA coating on day 2 and day 4, there was significant difference between the two, the ALP activity in Si-HA coating soaking decreased more than it in HA coating, there was significant difference between the two, scanning electron microscopy and staining suggested that more osteoblasts added in Si-HA coating. Conclusions. After the introduction of silicon into HA coating, the crystal structure and surface charge of the coating changed, it induced osteoblasts secreted collagen, Si-HA coating promoted the adherent growth and proliferation of osteoblasts. It may have good prospects in clinical orthopedic implants＇ surface modification.