海参精囊提取物对环磷酰胺诱导生殖系统受损小鼠 生精功能的影响*海参精囊提取物对环磷酰胺诱导生殖系统受损小鼠 生精功能的影响*

目的：本文旨在探讨海参精囊提取物对环磷酰胺诱导的生殖系统受损小鼠生精功能的保护作用。方法：昆明小白鼠随机分组，除正常组用生理盐水外其他各组均腹腔注射环磷酰胺（CP，28mg／kg），每天1次，连续5天，复制生殖系统受损、雄激素部分缺乏模型；同时治疗组每天灌胃不同剂量的药物1次，连续4周，记录小鼠的体重并观察其精神状态。计算精子总数、运动率、畸形率及睾丸组织中T-S0D酶活力，并与模型组进行比较。结果：相较于模型组小鼠，虽海参精囊提取物高低剂量间无明显差异（P〉0．05），但治疗组小鼠的精子总数、运动率、畸形率和T-SOD酶活力均有明显的改善（P〈0．01），甚至接近或高于正常水平。结论：海参精囊提取物对环磷酰胺导致的生殖系统受损小鼠的生精功能具有一定的保护作用。

Effects of Holothurian Spermatophore Extracts on Spermatogenesis of Reproductive Systemin Mice Injured by Cyclophosphamide*

Objective： To investigate the protective effects ofholothurian spermatophore extracts on spermatogenesis of the mice reproductive system injured by cyclophosphamide （CP）. Methods： Male Kunming mice were equally allotted to groups, and they were intraperitoneally injected with CP （28 mg/kg） once a day for 5 days to construct injured reproductive system and partial androgen deficie- ncy model except that the control group were intraperitoneally injected with normal saline. Mice in treatment groups orally received extracts once a day for 4 weeks. Record the changes of body weight and sprit state. Quantity, motility rate and abnormal rate of sperm and enzyme activity of T-SOD were determined and compared with those of model group. Results： Compared with that in model group, though the dose ofholothurian spermatophore extracts had no obvious difference （P〉0.05）, the quantity, sportive rate and abnormal rate of sperm and T-SOD enzyme activity were all significantly improved （P〈0.01）, even close to or higher than the normal level. Conclusion： Holothttfian spermatophore extracts had a protective effect on the spermatogenic lesion of reproductive system in mice injured by CP.