变应性鼻炎患者外周血中Tr1/Th3细胞的检测和分析

目的:检测变应性鼻炎(Allergic rhinitis,AR)患者和健康对照者外周血中IL-10+CD4+T细胞、TGF-β+CD4+T细胞(分别代表Tr1细胞和Th3细胞的特性)的比例,并探讨其在AR发病中的意义,为AR的治疗提供临床参考。方法:分离19例对粉尘螨过敏的AR患者和19例健康对照者外周血单个核细胞(PBMCs),采用流式细胞术分别检测外周血中IL-10+CD4+T细胞、TGF-β+CD4+T细胞的比例。结果:同健康对照者相比,AR患者外周血中IL-10+CD4+T细胞的比例显著降低(1.66±0.48)%vs.(3.80.92)%,t=-9.08,P0.01)],AR患者外周血中TGF-β+CD4+T细胞的比例降低(1.92±0.54)%vs.(4.76±1.12)%,t=-9.94,P0.01)]。结论:外周血中IL-10+CD4+T(Tr1)细胞比例的降低可能是AR发病的一个重要因素,提高AR患者外周血中分泌IL-10的Tr1细胞的比例可能在AR的治疗中具有重要意义。外周血中TGF-β1+CD4+T(Th3)细胞的比例显著降低,可能是AR发病的一个重要因素。但TGF-β1与AR关系的研究较少,特别是外周血中TGF-β1水平与AR的关系研究较少,需进一步研究。

Detection and Analysis of Tr1/Th3 cells in Peripheral Blood of Patients with Allergic Rhinitis

Objective:To detect the proportion of IL-10+CD4+T cells and TGF-beta+CD4+T cells (which represent characteristics of Tr1 cells and Th3 cells, respectively) in peripheral blood of allergic rhinitis (AR) patients and healthy controls, and to explore their significance in the pathogenesis of AR in order to provide clinical references for the treatment of allergic rhinitis.Methods:Peripheral blood mononuclear cells (PBMCs) of 19 AR patients who were sensed to Dermatophagoides farinae (Df) and 19 healthy controls were isolated. The proportion of IL-10+CD4+T cells and TGF-beta+CD4+T cells of peripheral blood were detected by flow cytometry.Results:Comparing with healthy controls'' the proportion of IL-10+CD4+ T cells in peripheral blood CD4+T cells significantly decreased and the TGF-beta1+ CD4+T cells in peripheral blood significantly decreased (P<0.01).Conclusion:A lower proportion of IL-10+CD4+ (Tr1) cells in peripheral blood may be an important factor in the pathogenesis of AR. Improve the proportion of IL-10 secreting Tr1 cells may be of great significance in the treatment of AR. A lower proportion of TGF-beta1+CD4+T (Th3) cells in peripheral blood may be an important factor in the pathogenesis of AR. However, few studies about the relationship between TGF-beta1 and AR, especially the relationship between TGF-beta1 levels and AR in peripheral blood, further research is needed.