MDA-DHPLC技术在PGS中的应用

目的:评估丙二醛变性高效液相色谱法在胚胎移植过程中的临床价值。方法:收集我院生殖中心2010年2月~2012年3间未做移植的废弃胚胎,由D2胚胎培养到D3胚胎期,提取遗传物质并进行PRC扩增,采用高形态学评分的D3作为对照,高效液相色谱法测定遗传DNA过氧化产物丙二醛(MDA)的含量。结果:多核Ⅰ组D2期卵裂球核≥2个,共24枚胚胎,DNA过氧化产物丙二醛(MDA)的含量5.32±0.19μmol/L,对比正常胚胎0.67±0.08μmol/l有显著差异(P0.05)。多核Ⅱ组D2期卵裂球核=2,共19枚胚胎,MDA的含量4.12±0.22μmol/L,对比正常胚胎0.67±0.08μmol/l有显著差异(P0.05)。Ⅰ组与Ⅱ组间没有统计学差异。然而,空泡胚胎与非空泡胚胎之间MDA并没有差异。结论:多核D2期胚胎培养到D3期会产生很高异常率,因此临床上应减少使用此种进行移植。

Application Research ofMDA-DHPLCin Preimplantation Genetic Screening*

Objective： To assess the application of MDA-DHPLC in the process of Embryo transferation. Methods： Select the abandoned D2 embryo from reproductive center of our hospital between the period of Feb.2010 to Jan.2012. Detect the content of the Peroxidation products MDA on DNA by DHPLC, take the good morphology D3 group as control. Results： There were 24 embryos in group Ⅰ whose D2 embryo is Multinucleated and blastomere nucleus ≥ 2, Peroxidation products MDA on DNA detected by DHPLC was 5.32 ± 0.19 μmol/l, compared with the controlled group 0.67± 0.08 μmol/L（P〈0.05）; 24 D2 embryos in group Ⅱ were Multinucleated and blastomere nucleus=2, Peroxidation products MDA was 4.12±0.22 μmol/L, compared with the controlled group 5.32± 0.19μmol/1 （P〈0.05）. There were no statistical deference between group Ⅰ and group Ⅱ （P〉0.05）. Neither between Multinucleated Vacuoles embryos and non-Vacuoles embryos. Conclusion： Development of Multinucleated D2 embryos to D3 derives high abnormal rates, we avoid to use those embryos to conduct Embryo transplantation.