喉癌组织中类高内皮微静脉形态学及蛋白多糖表达的研究

目的:通过对喉癌组织中类高内皮微静脉的分布、超微结构和蛋白多糖表达的观察,探讨淋巴细胞归巢的抗肿瘤意义及蛋白多糖对淋巴细胞归巢的调节作用。方法:应用透射电镜及阳性胶体铁染色方法,观察和研究32例喉癌组织中的类高内皮微静脉的分布、超微结构和蛋白多糖的表达。结果:(1)早期病变肿瘤周边组织内可见大量类高内皮微静脉,其内皮细胞高大,胞浆突起增多,细胞核大,细胞器丰富,有大量淋巴细胞穿越管壁,可见大量肿瘤浸润淋巴细胞存在;晚期病变组织内类高内皮微静脉少见,淋巴细胞穿越管壁不活跃,肿瘤浸润淋巴细胞明显减少。(2)类高内皮微静脉壁,特别是邻近管腔侧与阳性胶体铁呈强阳性反应,在有淋巴细胞穿越的部位反应更加明显,而内皮扁平的血管基本不反应或反应微弱。结论:(1)喉癌组织中部分毛细血管后微静脉可演变为类高内皮微静脉,是淋巴细胞向癌组织中浸润(淋巴细胞归巢)的重要场所;(2)肿瘤组织中的淋巴细胞归巢与抗肿瘤密切相关;(3)蛋白多糖于淋巴细胞归巢旺盛的类高内皮微静脉呈强表达,可能对归巢淋巴细胞穿越类高内皮微静脉有调节作用。

Study on the Morphology of High EndotheliumVenule-like Blood Vessels and Expression of Proteoglycan in Laryngeal Carcinoma

Objective:To observe the distribution and ultrastructure of high endothlium venule-like blood vessels in human laryngeal carcinoma and expression of proteogly and to inquire regulatory role of proteoglycans and the anticancer significance of lymphocyte homing.Methods:The distribution and ultrastructure of high endothelium venule-like blood vessels and expression of proteoglycan were studied by cationic colloid iron staining and electron microscopy in thirty-two human laryngeal carcinoma.Results:(1) Beside the laryngeal carcinoma, there were a lot of high endothelium venule-like blood vessels. Their endothelium became high. The organelles in endothelium were abundant, many cytoplasm projects were found on the endothelium surface. The nucleu was big. There were a large number of lymphocytes traverse through the wall of the high endothelium venule-like blood vessels. A large number of tumor infiltrating lymphocytes could be found in the laryngeal carcinoma tissue. High endothelial vein-like blood vessels were rarely observed in the latter stage of laryngeal carcinoma. Lymphocytes were not actively cross the wall of the high endothelium venule-like blood vessels, tumor infiltrating lymphocytes decreased significantly. (2) The wall of high endothelium venule-like blood vessels, especially the side next to the lumen of the vessels, was strongly positive staining. The parts with the lymphocytes traversing endothelium were stained stronger. The vessels with flat endotheliumwere negative staining.Conclusion:(1) Postcapillary venules can turn into high endothelium venule-like blood vessels in laryngeal carcinoma. They were the important site of lymphocyte homing.(2) The lymphocyte homing in laryngeal carcinoma had a closed relationship with their anticancer role. (3)Expression of proteoglycan were very strong in high endothelium venule-like blood vessels where the lymphocyte homing were active. Proteoglycan maybe regulate the homing of the tumor infiltrating lymphocyte.