针刺对缺氧缺血性脑损伤大鼠脑神经功能及5-HTR1A/cAMP/PKA信号通路的影响机制研究

摘要 目的：探讨与研究针刺对缺氧缺血性脑损伤大鼠脑神经功能及5-HTR1A/cAMP/PKA信号通路的影响机制。方法：研究时间为2022年6月到2022年12月，SPF级健康雄性SD大鼠36只平分为空白组、模型组、针刺组，每组各12只大鼠。空白组不进行造模，针刺组在造模完成1周后进行针刺治疗，空白组、模型组不进行治疗。结果：所有大鼠都顺利完成实验，无死亡大鼠出现。针刺组、模型组治疗后2周与4周的神经功能评分都显著高于空白组（P<0.05），针刺组的神经功能评分与模型组相比显著降低(P<0.05)。针刺组、模型组治疗后2周与4周的脑缺氧缺血组织体积都高于空白组(P<0.05)，针刺组的脑缺氧缺血组织体积与模型组相比显著降低（P<0.05）。针刺组、模型组治疗后2周与4周的血清超氧化物歧化酶活力低于空白组（P<0.05），血清丙二醛含量高于空白组（P<0.05），针刺组与模型组对比也有显著差异（P<0.05）。针刺组、模型组治疗后2周与4周的大脑组织5-HTR1A蛋白、cAMP蛋白、PKA蛋白相对表达水平明显低于空白组（P<0.05），针刺与模型组相比显著提高（P<0.05）。结论：针刺在缺氧缺血性脑损伤大鼠的应用能激活5-HTR1A/cAMP/PKA信号通路，能提高超氧化物歧化酶活力，降低血清丙二醛含量，能改善大鼠的脑神经功能，降低脑缺氧缺血组织体积。

Effect of Acupuncture on Brain Nerve Function and 5-HTR1A/cAMP/PKA Signal Pathway in Rats with Hypoxic-ischemic Brain Injury

ABSTRACT Objective: To explore and study the mechanism of acupuncture''s influence on brain nerve function and 5-HTR1A/cAMP/PKA signal pathway in rats with hypoxic-ischemic brain injury. Methods: The study period were from June 2022 to December 2022. 36 cases of SPF grade healthy male SD rats were equally divided into blank group, model group and acupuncture group, with 12 rats in each groups. The blank group were not subject to model building, the acupuncture group were subject to acupuncture treatment one week after the completion of model building, and the blank group and model group were not subject to treatment. Results: All rats were successfully completed the experiment and no dead rats were found. The scores of nerve function in acupuncture group and model group were significantly higher than that in blank group at 2 and 4 weeks after treatment (P<0.05), and the scores of nerve function in acupuncture group were significantly lower than that in model group(P<0.05). The volume of cerebral hypoxic-ischemic tissue in the acupuncture group and the model group at 2 and 4 weeks after treatment were higher than that in the blank group(P<0.05), and the volume of cerebral hypoxic-ischemic tissue in the acupuncture group were significantly lower than that in the model group (P<0.05). The activity of serum superoxide dismutase in the acupuncture group and the model group were lower than that in the blank group (P<0.05), and the content of serum malondialdehyde were higher than that in the blank group (P<0.05). There were also significant difference compared between the acupuncture group and the model group(P<0.05). The relative expression levels of 5-HTR1A protein, cAMP protein and PKA protein in the brain tissue of the acupuncture group and the model group were significantly lower than those of the blank group (P<0.05), and the acupuncture group were significantly higher than the model group(P<0.05). Conclusion: Acupuncture can activate the 5-HTR1A/cAMP/PKA signal pathway in rats with hypoxic-ischemic brain injury, increase the activity of superoxide dismutase, reduce the content of malondialdehyde in serum, improve the brain nerve function of rats, and reduce the volume of hypoxic-ischemic brain tissue.